Anti-SLIT1 Rabbit Polyclonal Antibody for WB, ELISA - O75093

Core Product Specifications and Parameters

Biological Background: SLIT1 Function and Localization

• SLIT1 encodes Slit homolog 1 protein, a large secreted glycoprotein containing multiple EGF-like domains and leucine-rich repeats (LRRs), belonging to the Slit family of guidance cues.
• It functions as a molecular guidance cue in cellular migration, mediating its effects through interaction with roundabout (Robo) homolog receptors.
• During neural development, SLIT1 is critical for axonal navigation at the ventral midline of the neural tube, directing axon projection to various brain regions.
• Together with SLIT2, it serves as a repulsive signal in the forebrain, preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
• The protein is predicted to be secreted and acts in the extracellular environment to guide migrating cells and axons.
• SLIT1 is predominantly expressed in the adult forebrain and also detected in fetal brain, lung, and kidney, indicating roles beyond neural development.
• Post-translational modifications include glycosylation and the formation of disulfide bonds, which stabilize its multiple EGF-like repeats.
• Alternative splicing generates multiple isoforms, potentially modulating its guidance functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1315-1534 of human SLIT1, a region likely within the C-terminal portion. Consider this when predicting epitope accessibility in native versus denaturing conditions.
• For Western blot (WB), the expected molecular weight is approximately 168 kDa. Use appropriate protein ladders and verify band specificity with relevant controls (e.g., knockout or siRNA-treated lysates).
• This polyclonal antibody may exhibit lot-to-lot variability; it is advisable to validate each new lot in the intended assay system.
• Optimal working concentrations for WB and ELISA should be determined empirically by the end user in their specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw-material sourcing, including this SLIT1 polyclonal antibody for neuroscience and developmental biology applications. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support rare target material procurement, bridging the gap from concept to clinical development.