Anti-SLIRP Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9GZT3

Core Product Specifications and Parameters

Biological Background: SLIRP Function and Localization

• SLIRP (SRA stem-loop-interacting RNA-binding protein, mitochondrial) is a 12 kDa RNA-binding protein that acts as a nuclear receptor corepressor, repressing SRA-mediated coactivation of nuclear receptors such as glucocorticoid (GR), androgen (AR), thyroid (TR), and vitamin D (VDR) receptors.
• The protein specifically binds the STR7 loop of SRA RNA, an lncRNA scaffold involved in transcriptional regulation, thereby modulating nuclear receptor signaling.
• SLIRP localizes to both the mitochondrion and the nucleus, suggesting dual roles in mitochondrial RNA metabolism and nuclear transcription regulation.
• It is ubiquitously expressed, with highest levels in heart, liver, skeletal muscle, and testis, indicating broad regulatory functions.
• The protein undergoes alternative splicing and phosphorylation, adding layers of post-translational control over its activity and localization.
• Functional annotations include roles as a repressor, RNA-binding protein, and transcription coregulator, highlighting its integration into steroid receptor signaling and mitochondrial gene expression.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide covering amino acids 1-100 of human SLIRP (NP_112487.1). This N-terminal region may be conserved, but verify specificity in your target species and experimental system.
• For Western blot (WB), a band around 12 kDa is expected; consider including appropriate molecular weight markers and positive controls (e.g., human heart or liver lysates).
• In immunohistochemistry (IHC-P), signal may be detectable in both nucleus and mitochondria; including antigen retrieval and blocking steps can help reduce background.
• For ELISA, use the peptide immunogen as a positive control if available, and validate linearity in your assay conditions.
• Since SLIRP is expressed across tissues, a knockout or knockdown control may be needed to confirm antibody specificity in functional studies.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio supports your development of diagnostic assays targeting nuclear receptor signaling, mitochondrial dysfunction, and SRA-mediated transcription. We offer validated antibody pairs, optimized polyclonal antibodies like this anti-SLIRP antibody, and bulk ancillary reagents, along with rare target and custom raw-material sourcing. Contact us today to accelerate your research and diagnostic manufacturing pipeline.