Anti-SLFN11 Rabbit Polyclonal Antibody for WB, ELISA - Q7Z7L1

Core Product Specifications and Parameters

Biological Background: SLFN11 Function and Localization

• SLFN11 is a member of the schlafen family, acting as an inhibitor of DNA replication and promoting cell death upon DNA damage (PubMed:22927417, PubMed:26658330, PubMed:29395061). Related references: PMID:22927417 PMID:26658330 PMID:29395061
• It functions as a guardian of the genome by killing cells with defective replication, persistently blocking stressed replication forks by opening chromatin to inhibit fork progression (PubMed:29395061). Related references: PMID:29395061
• Upon DNA damage, SLFN11 inhibits translation of ATR or ATM based on distinct codon usage without disrupting early DNA damage response signaling (PubMed:30374083). Related references: PMID:30374083
• SLFN11 is an antiviral restriction factor with manganese-dependent type II tRNA endoribonuclease activity, cleaving a single tRNA molecule in a dimeric complex (PubMed:36115853). Related references: PMID:36115853
• It specifically abrogates retrovirus production, including HIV-1, and impairs replication of human cytomegalovirus and some Flaviviruses by exploiting viral codon bias towards A/T nucleotides (PubMed:23000900, PubMed:35105802, PubMed:36115853). Related references: PMID:23000900 PMID:35105802 PMID:36115853
• SLFN11 localizes primarily to the nucleus and chromosomes.
• It exhibits wider expression in ovarian and colon adenocarcinoma than in corresponding healthy tissues.
• Key functional domains and keywords include ATP-binding, DNA-binding, endonuclease, helicase, hydrolase, and tRNA-binding.

Experimental Guidance and Technical Tips

• For Western blot, consider denaturing and reducing conditions given the predicted molecular weight of 103 kDa.
• Validate antibody specificity using positive and negative cell lines that express SLFN11, such as ovarian or colon cancer cells.
• For ELISA, test the antibody in direct or sandwich formats using recombinant SLFN11 protein or the immunogen as capture antigen.
• Optimize antibody dilution and incubation times empirically, as polyclonal antibodies can vary by lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. The SLFN11 Rabbit Polyclonal Antibody is a valuable tool for investigations into DNA damage response, viral restriction, and oncology. CamelBio's supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials to accelerate your assay development and validation.