Anti-SLFN11 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - Q7Z7L1

Core Product Specifications and Parameters

Biological Background: SLFN11 Function and Localization

• SLFN11 is a member of the Schlafen family that functions as a guardian of the genome by inhibiting DNA replication and promoting cell death in response to DNA damage. It persistently blocks stressed replication forks by opening chromatin at initiation sites, possibly unwinding DNA ahead of the MCM helicase (PubMed:29395061, PubMed:22927417, PubMed:26658330). Related references: PMID:22927417, PMID:26658330, PMID:29395061
• Upon DNA damage, SLFN11 inhibits translation of ATR or ATM based on distinct codon usage without disrupting early DNA damage response signaling (PubMed:30374083). Related references: PMID:30374083
• SLFN11 acts as an antiviral restriction factor with manganese-dependent type II tRNA endoribonuclease activity. A single tRNA molecule is bound and cleaved by the SLFN11 dimer (PubMed:36115853). Related references: PMID:36115853
• It specifically abrogates the production of retroviruses like HIV-1 by inhibiting protein synthesis in a codon-usage-dependent manner, exploiting the viral codon bias towards A/T nucleotides (PubMed:23000900). Related references: PMID:23000900
• SLFN11 also impairs the replication of human cytomegalovirus (HCMV) and some Flaviviruses (PubMed:35105802, PubMed:36115853). Related references: PMID:35105802, PMID:36115853
• Localized in the nucleus and associated with chromosomes, SLFN11 is an interferon-induced protein with broader expression in ovarian and colon adenocarcinomas compared to healthy tissues.
• Key features include ATP-binding, DNA-binding, RNA-binding, tRNA-binding, helicase, endonuclease, and hydrolase activities, highlighting its multifunctional role in immunity and genomic stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 132–328 of human SLFN11; consider validating the antibody in relevant human cell lines expressing full-length SLFN11.
• For Western blotting, a band at approximately 103 kDa is expected; include appropriate positive and negative controls.
• In immunofluorescence/ICC, anticipate nuclear/chromosomal staining; optimize fixation and permeabilization conditions for your sample type.
• For immunoprecipitation, empirically optimize antibody and lysate concentrations; validate elution conditions to preserve SLFN11 integrity.
• ELISA may be useful for quantitative detection; ensure proper blocking and washing steps to minimize background.
• Since cross-reactivity is reported only for human, avoid use in other species unless independently validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For SLFN11 research in DNA damage, antiviral, and cancer studies, CamelBio ensures reliable supply and technical support to accelerate your assay development. Partner with us to streamline your raw-material procurement from concept to clinic.