Anti-SLCO1A2 Polyclonal Antibody for WB, ELISA - P46721

Core Product Specifications and Parameters

Biological Background: SLCO1A2 Function and Localization

• SLCO1A2 (OATP1A2, SLC21A3) is a member of the solute carrier organic anion transporter family, functioning as a Na+-independent transporter at the cell membrane.
• It mediates cellular uptake of endogenous bile salts, including cholate and deoxycholate in both unconjugated and conjugated forms, facilitating intestinal bile acid absorption. Related references: PMID:19129463 PMID:7557095
• The transporter accommodates a broad spectrum of organic anions, including steroid sulfates (DHEAS, estrone 3-sulfate), 17beta-estradiol 17-O-(beta-D-glucuronate), and clinically used drugs such as methotrexate and paclitaxel. Related references: PMID:11159893 PMID:12568656 PMID:19129463 PMID:17301733 PMID:20686826 PMID:27777271 PMID:23243220
• In the retinal pigment epithelium, SLCO1A2 mediates apical uptake of all-trans-retinol (Vitamin A), which is essential for maintaining the visual cycle. Related references: PMID:25560245
• It transports thyroid hormones (both T3 and T4) and prostaglandin E2, and contributes to hormone uptake by neural cells. Related references: PMID:19129463 PMID:20358049
• The protein displays pH-sensitive substrate specificity, with hydrogencarbonate (HCO3-) likely acting as the counteranion exchanged for organic anions. Related references: PMID:19129463
• Tissue distribution includes high expression in brain (cortex, hippocampus), liver, kidney, placenta, lung, testis (Sertoli cells), and retina, with weaker expression in prostate.
• Post-translational modifications include disulfide bonds and glycosylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 400-500 of human SLCO1A2; cross-reactivity with rat suggests potential reactivity with other species—validate in the relevant sample system.
• For Western blot, consider the predicted molecular weight of ~74 kDa; optimize gel percentage and transfer conditions accordingly.
• For ELISA, titrate the antibody under specific assay conditions to determine optimal coating and detection concentrations.
• As a multi-pass membrane protein, sample preparation may benefit from membrane protein extraction protocols to improve detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, supporting every stage from concept to clinic. For transporters like SLCO1A2, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, facilitating robust assay development for drug transport, hormone delivery, and bile acid homeostasis research. Our sourcing capabilities also extend to rare target raw materials, ensuring a reliable supply chain for your specialized IVD needs.