Anti-SLC9A6 Rabbit Polyclonal Antibody for WB and ELISA - Q92581

Core Product Specifications and Parameters

Biological Background: SLC9A6 Function and Localization

• The SLC9A6 gene encodes the sodium/hydrogen exchanger 6 (NHE6), a member of the solute carrier 9 (SLC9) family; alternative splicing produces multiple isoforms.
• NHE6 functions as an endosomal Na⁺/K⁺:H⁺ antiporter, mediating electroneutral exchange of luminal protons for cytosolic alkali ions to regulate intra-endosomal pH. Related references: PMID:15522866, PMID:28635961, PMID:31676550
• By counteracting vacuolar ATPase-driven acidification, NHE6 limits endosomal acidification, essential for proper endosome maturation and cargo trafficking. Related references: PMID:15522866, PMID:28635961, PMID:31676550
• The protein localizes to early, recycling, and late endosome membranes; in specialized cells such as osteoclasts, it also traffics to the plasma membrane.
• Expression is ubiquitous, with highest levels in brain, skeletal muscle, and heart; mutations are linked to intellectual disability, epilepsy, and Parkinsonism.
• Post-translational modifications include glycosylation and ubiquitination; the protein plays a role in maintaining cell polarity and synaptic development. Related references: PMID:20130086

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal region (amino acids 542–701), which may exhibit species-specific sequence variations; verify cross-reactivity by sequence alignment.
• For Western blotting, the expected band size is approximately 78 kDa; consider using reducing and non-reducing conditions to confirm specificity.
• ELISA protocols may require optimization of coating antigen concentration and detection reagents; test multiple conditions to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement solution for IVD raw materials, offering this anti-SLC9A6 antibody along with validated antibody pairs, bulk ancillary reagents, and custom monoclonal antibody development. Supporting neuroscience and ion transport research, we ensure reliable access to rare targets and comprehensive technical resources. From discovery to clinical application, CamelBio bridges the gap between your concepts and clinical reality.