Anti-SLC9A3R2 Polyclonal Antibody for WB, ELISA - Q15599

Core Product Specifications and Parameters

Biological Background: SLC9A3R2 (NHERF2) Function and Localization

• Also known as NHERF2, E3KARP, SIP-1, and TKA-1; encoded by the SLC9A3R2 gene; belongs to the NHERF family of PDZ domain-containing scaffold proteins.
• Functions as a scaffold that links plasma membrane proteins (e.g., SLC9A3) to the actin cytoskeleton via interactions with ezrin/moesin/radixin proteins, regulating their surface expression and localization.
• Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3, a sodium-hydrogen exchanger, as demonstrated in functional studies (PubMed:18829453). Related references: PMID:18829453
• May also function as a scaffold protein in the nucleus, suggesting additional roles in transcriptional regulation or nuclear organization.
• Localizes to the apical cell membrane, endomembrane system, and nucleus, indicating dynamic trafficking and multiple functional compartments.
• Undergoes alternative splicing and phosphorylation (as indicated by UniProt keywords), which may modulate its scaffolding activity or interactions.
• Widely expressed across human tissues, consistent with its general role in membrane protein regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 180-300 of human SLC9A3R2 (NP_001123484). This region may be conserved or specific; researchers should verify specificity in their experimental context.
• For Western blotting, the predicted molecular weight is approximately 37 kDa. Endogenous expression levels and post-translational modifications could result in additional bands; use appropriate controls.
• In ELISA applications, consider using the full-length recombinant protein or peptide as coating antigen to confirm antibody reactivity.
• For human samples, ensure that the sample processing maintains protein integrity, especially due to membrane localization (requires mild detergents).

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