Anti-SLC9A3R1 Rabbit Polyclonal Antibody for WB, ELISA - O14745

Core Product Specifications and Parameters

Biological Background: SLC9A3R1 Function and Localization

• SLC9A3R1, also known as NHERF1, EBP50, NHE-RF, NHERF, and NPHLOP2, is a 39 kDa scaffold protein belonging to the sodium-hydrogen exchanger regulatory factor family.
• Contains two PDZ domains and a C-terminal ezrin/radixin/moesin (ERM)-binding domain, enabling it to link plasma membrane proteins to the actin cytoskeleton.
• Regulates the activity and subcellular localization of several ion transporters, including the sodium-hydrogen exchanger SLC9A3 (NHE3) and the β2-adrenergic receptor ADRB2.
• Plays a role in Wnt signaling, phosphate reabsorption in renal proximal tubules, and chloride/bicarbonate homeostasis in spermatozoa.
• Subcellularly localized to the cytoplasm, apical cell membrane, microvilli, filopodia, and membrane ruffles.
• Undergoes post-translational modifications including phosphorylation and acetylation, and is subject to alternative splicing.
• Expressed in a variety of tissues including liver, kidney, pancreas, prostate, spleen, small intestine, and placenta, particularly in the syncytiotrophoblast.

Experimental Guidance and Technical Tips

• As a rabbit polyclonal antibody generated against a recombinant fragment (amino acids 189-358) of human SLC9A3R1, it is expected to recognize both full-length and potentially splice variants of NHERF1 across human, mouse, and rat.
• For Western blot, consider optimizing primary antibody dilution (e.g., 1:500–1:2000) based on sample expression levels and detection system. Validate in relevant cell or tissue lysates expressing the target.
• For ELISA, use standard direct or indirect protocols; include appropriate negative and positive controls. Perform checkerboard titrations to determine optimal coating antigen and antibody concentrations.
• Due to the scaffold protein's involvement in multi-protein complexes, ensure lysis conditions preserve interactions if analyzing native complexes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. With expertise spanning from concept to clinic, we source rare-target raw materials and support every stage of assay development. For SLC9A3R1 (NHERF1)-related studies, we offer customized solutions to meet your procurement needs.