Anti-SLC8A2 (NCX2) Rabbit Polyclonal Antibody for WB, ELISA - Q9UPR5

Core Product Specifications and Parameters

Biological Background: Sodium/calcium exchanger 2 Function and Localization

• SLC8A2 (Solute carrier family 8 member 2), also known as NCX2, is a member of the sodium/calcium exchanger family that mediates electrogenic exchange of Ca²⁺ for Na⁺ across the plasma membrane.
• It plays a critical role in rapidly clearing cytoplasmic Ca²⁺ after neuronal excitation, thereby modulating synaptic plasticity, learning, and memory processes.
• In the kidney, SLC8A2 contributes to the regulation of urinary Ca²⁺ and Na⁺ excretion, underscoring its role in systemic ion homeostasis.
• The protein localizes predominantly to the cell membrane, with enrichment at basolateral surfaces in polarized cells, and is also found in perikarya, dendrites, and dendritic spines of neurons.
• SLC8A2 contains multiple transmembrane domains and a large cytoplasmic loop that harbors regulatory sites, including a calmodulin-binding domain and phosphorylation residues.
• Post-translational modifications include glycosylation and phosphorylation, which may modulate exchanger activity and trafficking.
• As an antiporter, it is essential for maintaining low resting cytosolic Ca²⁺ in excitable cells and preventing Ca²⁺ overload, highlighting its neuroprotective potential.

Experimental Guidance and Technical Tips

• The immunogen spans a central cytoplasmic region (aa 360-450) that is unique to SLC8A2 among NCX family members; nevertheless, we recommend verifying specificity by siRNA knockdown or comparison with SLC8A1/SLC8A3 knockout lysates.
• For western blotting, the predicted molecular weight is ~100 kDa; use a 7.5–10% SDS-PAGE gel and optimize transfer conditions (e.g., wet transfer with 10% methanol) for high-molecular-weight proteins.
• The antibody is expected to detect the full-length protein; additional lower bands may represent proteolytic fragments or immature forms—validate with appropriate controls.
• ELISA performance depends on the coating antigen; use recombinant SLC8A2 extracellular or cytoplasmic domain fragments and optimize coating buffer and blocking conditions.
• Since SLC8A2 is predominantly expressed in brain and kidney, consider using tissue lysates from these sources for primary validation.
• For immunolocalization studies, note that native protein localizes to dendritic spines; ensure permeabilization and fixation protocols are compatible with membrane protein detection.

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CamelBio empowers diagnostic manufacturers and researchers with one-stop access to premium IVD raw materials, including validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents tailored for ion channel and neuroscience targets. For projects targeting calcium signaling or excitable cell biology, our supply chain network can source rare-target raw materials such as this anti-SLC8A2 antibody, streamlining your transition from concept to clinic. Partner with us to secure high-quality reagents with consistent performance and comprehensive technical support.