Anti-SLC6A8 Rabbit Polyclonal Antibody for WB and ELISA - P48029

Core Product Specifications and Parameters

Biological Background: SLC6A8 Function and Localization

• SLC6A8, also known as CRT, CT1, or CRT1, is a sodium- and chloride-dependent creatine transporter encoded by the SLC6A8 gene on chromosome X.
• Functions as a creatine:sodium symporter, mediating cellular uptake of creatine, which is critical for energy metabolism in tissues like brain and muscle. Related references: PMID:17465020, PMID:22644605, PMID:25861866
• Predominantly expressed in skeletal muscle and kidney, with lower levels in brain, heart, colon, testis, and prostate, reflecting its role in creatine distribution.
• Localized to the cell membrane, particularly the apical cell membrane, enabling directional creatine transport across epithelia and the blood-brain barrier.
• Contains 12 transmembrane helices typical of solute carrier family 6 members, and undergoes alternative splicing, phosphorylation, and glycosylation.
• Mutations in SLC6A8 are linked to cerebral creatine deficiency syndrome 1 (CCDS1), an X-linked intellectual disability disorder, highlighting its importance in brain creatine homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal cytoplasmic domain (aa 539–635), which is conserved between human and mouse, supporting the observed cross-reactivity.
• For Western blot, the predicted molecular weight is ~71 kDa; the glycosylated form may appear slightly higher. Validate band specificity using tissue lysates from brain or kidney.
• For ELISA, consider coating with the recombinant immunogen or native protein extracts; titrate the antibody to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibodies against key transporter proteins like SLC6A8. Our one-stop platform streamlines sourcing of antibody pairs, bulk reagents, and rare-target raw materials, supporting your diagnostic development from concept to clinic.