Anti-SLC6A5 (GLYT2) Polyclonal Antibody for WB and ELISA - Q9Y345

Core Product Specifications and Parameters

Biological Background: SLC6A5 (Glycine Transporter 2) Function and Localization

• SLC6A5 encodes the sodium- and chloride-dependent glycine transporter 2 (GlyT2), a solute carrier 6 family member, with alternative splicing producing functionally distinct isoforms.
• The full-length transporter catalyzes high-affinity sodium- and chloride-dependent glycine reuptake into presynaptic terminals, essential for terminating glycinergic neurotransmission at inhibitory synapses. Related references: PMID:9845349, PMID:10381548, PMID:10606742, PMID:16751771
• GlyT2 is localized to the plasma membrane, consistent with its role in synaptic neurotransmitter clearance.
• It is predominantly expressed in the medulla and, to a lesser extent, the spinal cord and cerebellum.
• The protein is a glycoprotein and phosphoprotein, containing disulfide bonds and metal-binding sites, which may regulate its trafficking and transport activity.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal cytoplasmic region (aa 1–200) that is highly conserved among human, mouse, and rat SLC6A5 orthologs, consistent with the manufacturer’s reported cross-reactivity.
• For Western blot analysis, expect a major band near 87 kDa; membrane-rich protein extracts are recommended for optimal signal.
• In ELISA, consider pairing with a monoclonal detection antibody to establish a quantitative assay; validate signal linearity and background in the relevant species matrix.
• As a polyclonal antibody, it may bind multiple epitopes, increasing sensitivity for low-abundance targets, but pre-adsorption or knockout control is advised to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies rigorously selected IVD raw materials to diagnostic manufacturers and researchers worldwide. This anti-SLC6A5 polyclonal antibody supports neuroscience and transporter research, and we offer complementary reagents such as validated antibody pairs, blocking buffers, and ancillary proteins for glycine transporter studies. Contact us for custom bulk orders or assistance in sourcing rare targets.