Anti-SLC6A2 (Norepinephrine Transporter) Rabbit Polyclonal Antibody for WB and ELISA - P23975

Core Product Specifications and Parameters

Biological Background: SLC6A2 Function and Localization

• Encoded by the SLC6A2 gene, the sodium-dependent noradrenaline transporter (NET) is a member of the solute carrier family 6 and mediates the reuptake of norepinephrine at sympathetic synapses (PubMed:2008212, PubMed:8125921, PubMed:38750358). Related references: PMID:2008212 PMID:8125921 PMID:38750358
• The transporter clears norepinephrine from the synaptic cleft, terminating its signaling and recycling the neurotransmitter for future release (By similarity).
• NET also transports dopamine with high affinity, contributing to catecholamine homeostasis in the brain and periphery (PubMed:11093780, PubMed:8125921, PubMed:39395208). Related references: PMID:11093780 PMID:8125921 PMID:39395208
• Subcellularly, NET localizes to the plasma membrane, particularly at axonal varicosities and presynaptic terminals, where it cycles between the membrane and intracellular compartments.
• The protein contains 12 transmembrane domains, extracellular N-glycosylation sites, and intracellular phosphorylation and palmitoylation sites that regulate its trafficking and activity (UniProt keywords: Glycoprotein, Lipoprotein, Palmitate, Transmembrane helix).
• Alternative splicing yields multiple isoforms, and polymorphisms in SLC6A2 have been linked to orthostatic intolerance, depression, and attention deficit hyperactivity disorder (ADHD) (UniProt keywords: Alternative splicing, Disease variant).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering the N-terminal extracellular/intracellular loop region (aa 30-235); this design favors recognition of native, non-denatured epitopes in applications like ELISA. For WB, consider using whole-cell lysates from tissues rich in sympathetic innervation (e.g., heart, adrenal gland, vas deferens) or transfected cell lines.
• As a polyclonal antibody, batch-to-batch consistency should be monitored; we recommend titrating the antibody for each new lot in your assay system. Endogenous NET is often detected as a glycosylated band around 69–80 kDa; deglycosylation enzymes can shift the band to confirm specificity.
• NET internalization can be influenced by experimental conditions (e.g., temperature, phorbol esters); to detect total protein, include membrane fraction enrichment and protease inhibitors during sample preparation.

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