Anti-SLC6A1 Polyclonal Antibody for WB, ELISA - P30531

Core Product Specifications and Parameters

Biological Background: SLC6A1 (GAT1) Function and Localization

• SLC6A1 encodes the sodium- and chloride-dependent GABA transporter 1 (GAT1), also known as solute carrier family 6 member 1, which is the primary GABA transporter in the brain responsible for reuptake of the inhibitory neurotransmitter GABA from the synaptic cleft (PubMed:30132828). Related references: PMID:30132828
• The transporter mediates the symport of GABA with two Na⁺ ions and one Cl⁻ ion, and its transport direction and magnitude are governed by the transmembrane ion gradients and membrane potential, allowing both forward (uptake) and reverse (release) GABA transport.
• SLC6A1 can also transport hypotaurine in a sodium- and chloride-dependent manner, albeit with much lower affinity compared to GABA.
• The protein localizes predominantly to the presynaptic membrane of GABAergic neurons, where it plays a critical role in terminating GABAergic signaling and maintaining neurotransmitter homeostasis.
• SLC6A1 is a multi-pass transmembrane glycoprotein and phosphoprotein, with its activity regulated by post-translational modifications including phosphorylation and the presence of a conserved disulfide bond.
• Mutations in SLC6A1 are associated with neurological disorders such as epilepsy, reflecting its essential role in inhibitory neurotransmission.
• The protein contains a metal-binding site and belongs to the sodium:neurotransmitter symporter family (SNF), characterized by 12 transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 500-599 of human SLC6A1, a region likely exposed on the intracellular side following predicted topology. Use this information for epitope-specific design consideration.
• This polyclonal antibody is indicated for Western blot (WB) and ELISA, with detected band expected at approximately 67 kDa. Consider using appropriate tissue or cell lysates expressing SLC6A1 (e.g., brain samples) and validate with controls.
• Demonstrates cross-reactivity with human, mouse, and rat SLC6A1 as per supplier specifications, making it suitable for studies in these species. Orthogonal confirmation via siRNA knockdown or overexpression is recommended.
• As a polyclonal preparation, lot-to-lot consistency should be monitored; include a reference standard if quantitative comparisons are needed.
• For WB, optimize blocking and antibody incubation conditions, and include positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including polyclonal antibodies like this anti-SLC6A1 (GAT1) product for neuroscience and GABA transporter studies. With capabilities spanning validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, CamelBio acts as a reliable procurement bridge from concept to clinic. Researchers can leverage our integrated supply chain and technical services to streamline development of neurotransmitter-related assays.