Anti-SLC5A9 Rabbit Polyclonal Antibody for WB and ELISA - Q2M3M2

Core Product Specifications and Parameters

Biological Background: SLC5A9/SGLT4 Function and Localization

• SLC5A9 (SGLT4) is a member of the solute carrier family 5 and encodes a sodium/glucose cotransporter 4 that facilitates electrogenic Na⁺-coupled sugar symport.
• The transporter specifically recognizes sugar substrates with a pyranose ring and an axial hydroxyl group on carbon 2, showing preference for D-mannose and possibly D-fructose and D-glucose.
• Its transport activity is driven by a transmembrane Na⁺ electrochemical gradient maintained by the Na⁺/K⁺ pump.
• SLC5A9 is localized to the cell membrane and is expressed in metabolically relevant tissues including the small intestine, kidney, and liver.
• The protein undergoes post-translational modifications such as glycosylation and phosphorylation, which may regulate its function and localization.
• Alternative splicing of SLC5A9 produces multiple isoforms that may have distinct functional properties.
• Keywords from UniProt include: Alternative splicing, Cell membrane, Glycoprotein, Ion transport, Membrane, Phosphoprotein, Proteomics identification, Reference proteome, Sodium, Sodium transport, Sugar transport, Symport, Transmembrane, Transmembrane helix, Transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region (aa 230–320) of human SLC5A9, which is conserved in human and mouse; ensure reactivity is validated in relevant species and tissue types.
• For Western blot, a band around 74 kDa is expected; consider optimizing blocking and antibody dilution using lysates from SLC5A9-expressing tissues (small intestine, kidney, liver).
• For ELISA, use recombinant SLC5A9 protein or peptide as the coating antigen and include appropriate positive and negative controls.
• Cross-reactivity with other SLC5 family members or isoforms cannot be excluded; verify specificity in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, supporting every stage from concept to clinic. This Anti-SLC5A9 antibody is part of our portfolio for ion transport and metabolic research. We offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your assay development.