Anti-SLC5A7 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9GZV3

Core Product Specifications and Parameters

Biological Background: SLC5A7 Function and Localization

• SLC5A7 encodes the high affinity choline transporter 1 (CHT1/CHT), a Na(+)- and Cl(-)-dependent transmembrane symporter that mediates choline uptake into presynaptic neurons with electrogenic, voltage-dependent kinetics (PubMed:11027560, PubMed:11068039, PubMed:12237312, PubMed:17005849). Related references: PMID:11027560 PMID:11068039 PMID:12237312 PMID:17005849
• This transporter catalyzes the rate-limiting step of acetylcholine (ACh) synthesis by recovering choline from the synaptic cleft, thereby sustaining cholinergic neurotransmission and cognitive functions (PubMed:11027560, PubMed:27569547). Related references: PMID:11027560 PMID:27569547
• CHT1 predominantly localizes to intracellular presynaptic organelles (e.g., early endosomes, synaptic vesicles) and rapidly translocates to the axonal plasma membrane in response to neuronal activity to boost choline import (PubMed:12969261, PubMed:15953352). Related references: PMID:12969261 PMID:15953352
• Expression is prominent in cholinergic neurons of the putamen, spinal cord, and medulla, mirroring regions critical for motor control and autonomic regulation (PubMed:11027560, PubMed:11068039, PubMed:27569547). Related references: PMID:11027560 PMID:11068039 PMID:27569547
• Loss-of-function mutations in SLC5A7 underlie congenital myasthenic syndrome (CMS20) and distal hereditary motor neuropathy (HMN7A), highlighting its essential role in neuromuscular junction maintenance.
• The transporter is a glycoprotein and phosphoprotein, suggesting regulation by post-translational modifications that may influence trafficking and activity.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal cytoplasmic domain (aa 503–580), which may favor recognition of the full-length transporter and minimize interference with substrate binding or ligand interactions.
• For Western blot (WB), a dilution series is advisable to optimize signal; the predicted molecular weight is approximately 63 kDa, though glycosylation may shift apparent mobility.
• In immunofluorescence (IF-P), careful fixation and permeabilization are critical because the protein resides predominantly in intracellular vesicles and presynaptic terminals, with only a fraction exposed on the plasma membrane.
• Cross-reactivity with mouse and rat samples allows for translational studies, but validate the antibody in the relevant tissue or cell system before large-scale experiments.
• The antibody can be used in ELISA-based assays after confirming specificity; consider including appropriate positive (e.g., brain lysate) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner for diagnostic manufacturers and research labs, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For investigations into cholinergic neurotransmission and transporter biology, we facilitate sourcing of anti-SLC5A7 antibodies, matching antigen controls, and related rare-target reagents. With a supply chain spanning concept to clinic, CamelBio streamlines procurement and technical support for your assay development needs.