Anti-SLC5A3 Polyclonal Antibody for WB, ELISA - P53794

Core Product Specifications and Parameters

Biological Background: SLC5A3 Function and Localization

• SLC5A3 encodes the sodium/myo-inositol cotransporter (SMIT1), a member of the solute carrier family 5, which actively transports myo-inositol and scyllo-inositol across the plasma membrane using the Na+ gradient.
• It maintains cellular myo-inositol concentration, crucial for cell volume regulation and fluid balance during osmotic stress, particularly in the fetoplacental unit and central nervous system.
• The transporter forms coregulatory complexes with voltage-gated K+ channels (e.g., KCNQ1-KCNE2, KCNQ2-KCNQ3), allosterically modulating ion selectivity and gating; this interplay influences neuronal excitability and myo-inositol gradients in the choroid plexus. Related references: PMID:24595108, PMID:28793216
• SLC5A3 provides myo-inositol for phosphoinositide synthesis (e.g., PI(4,5)P2), thereby indirectly regulating phosphoinositide-dependent ion channels and Ca2+ signaling during osmotic stress. Related references: PMID:27217553
• The protein localizes to both the apical and basolateral cell membranes, consistent with its role in transepithelial solute transport.
• Post-translational modifications include glycosylation and phosphorylation, as annotated by the keywords Glycoprotein and Phosphoprotein.
• Alternative names include BCW2, SMIT, SMIT1, SMIT2, reflecting its sodium/myo-inositol transporter function and gene family.
• The predicted molecular weight is approximately 79.7 kDa (UniProt), consistent with the observed 80 kDa band in western blot.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 535–695), so the antibody is expected to recognize epitopes within the intracellular domain. For western blot, use reducing conditions and load adequate protein; a band around 80 kDa should be detected.
• When performing ELISA, consider pairing this antibody with a capture antibody targeting a distinct epitope if developing a sandwich assay; validate with recombinantly expressed SLC5A3 or tissue lysates known to express the transporter.
• For species cross-reactivity, positive signals are reported in human, mouse, and rat. Validate in your specific sample system; suitable positive controls include kidney, brain, or placental tissues where SLC5A3 is expressed.
• This polyclonal antibody may exhibit batch-to-batch variation typical of polyclonal reagents; consider including a reference standard in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between concept and clinic by offering diagnostic manufacturers, laboratories, and research institutes one-stop access to IVD raw materials, technical services, and consulting. This anti-SLC5A3 polyclonal antibody supports research into sodium/myo-inositol cotransport, osmotic stress, and ion channel regulation. Beyond this product, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to streamline your assay development pipeline.