Anti-SLC5A2 Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - P31639

Core Product Specifications and Parameters

Biological Background: SLC5A2 Function and Localization

• SLC5A2 encodes the sodium/glucose cotransporter 2 (SGLT2), a member of the solute carrier family 5, functioning as a low-affinity electrogenic Na+/glucose symporter.
• The transporter actively mediates D-glucose uptake across the plasma membrane with a 1:1 Na+ to sugar stoichiometry, driven by the transmembrane Na+ electrochemical gradient. Related references: PMID:20980548 PMID:28592437 PMID:34880493
• Full transporter activity requires interaction with the auxiliary protein PDZK1IP1/MAP17. Related references: PMID:37217492
• SLC5A2 is predominantly expressed in the kidney, where it plays a critical role in reabsorbing glucose from the glomerular filtrate in the early proximal tubules.
• Subcellularly, SGLT2 localizes to the apical cell membrane of renal proximal tubular epithelial cells.
• The protein is a glycoprotein with multiple transmembrane helices and disulfide bonds, and alternative splicing generates multiple isoforms; mutations are linked to renal glucosuria.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 564-624) of human SLC5A2. Consider this when designing blocking peptides or competition assays.
• For Western blot, a band near 73 kDa is expected, but glycosylation may cause a shift; validate with relevant controls (e.g., deglycosylation enzymes) if needed.
• In immunohistochemistry (IHC-P), use FFPE kidney sections with appropriate antigen retrieval. For immunofluorescence (IF-P), frozen sections or cultured renal cell lines are recommended.
• Cross-reactivity with mouse and rat is indicated; however, always verify specificity in your sample system using positive and negative controls.
• For ELISA applications, standard coating and detection protocols can be adopted, but optimize antibody dilution individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials. As a one-stop partner, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents tailored to renal biomarker research. From target selection to clinical validation, CamelBio bridges your sourcing needs—whether you require rare target reagents or scalable production for routine assays.