Anti-SLC5A1 Rabbit Polyclonal Antibody for WB, ELISA - P13866

Core Product Specifications and Parameters

Biological Background: SLC5A1 Function and Localization

• SLC5A1 encodes the sodium/glucose cotransporter 1 (SGLT1), a high-affinity, Na+-dependent symporter of the solute carrier family 5.
• The protein mediates electrogenic co-transport of D-glucose or D-galactose with Na+ at a 2:1 stoichiometry, driven by the transmembrane Na+ gradient (PubMed:20980548, 34880492, 35077764). Related references: PMID:20980548, PMID:34880492, PMID:35077764
• In enterocytes, SGLT1 is the principal apical transporter for dietary glucose and galactose absorption; basolateral exit is mediated by GLUT2.
• Functions as a glucose sensor in enteroendocrine cells, triggering secretion of the incretins GIP and GCG to regulate appetite and energy homeostasis (PubMed:8563765). Related references: PMID:8563765
• In the kidney, SGLT1 participates in glucose reabsorption in the S3 segment of proximal tubules, complementing SGLT2-mediated reabsorption.
• Expressed in endometrial epithelial cells, where it imports glucose for glycogen synthesis and supports embryo nutritional needs and decidual transformation (PubMed:28974690). Related references: PMID:28974690
• Also functions as a passive water channel with aquaporin-like conductivity, contributing to transepithelial water flux in the small intestine.
• Localizes to the apical plasma membrane and is subject to glycosylation and phosphorylation; alternative splicing generates additional isoforms.

Experimental Guidance and Technical Tips

• The immunogen targets a synthetic peptide within the intracellular loop (aa 400-500); this region may be well-exposed for antibody binding.
• For western blotting, the predicted molecular weight is approximately 73 kDa; validate using lysates from human intestinal or endometrial tissue/cell lines.
• For ELISA, consider peptide blocking experiments to confirm signal specificity.
• Recommended applications include WB and ELISA based on supplier data; additional applications may require individual optimization.
• This product shows reactivity with human SLC5A1; cross-reactivity with other species should be verified experimentally.
• Shipment on ice bag indicates temperature sensitivity; store at recommended conditions upon receipt.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research groups a streamlined, single-vendor solution for IVD raw materials and technical support. For glucose transporter research, this SLC5A1 polyclonal antibody is part of our portfolio targeting metabolic and renal biomarkers. We facilitate procurement of validated antibody pairs, recombinant proteins, and ancillary reagents—bridging the gap from early assay development to clinical-grade production.