Anti-SLC4A8 Rabbit Polyclonal Antibody for WB, ELISA - Q2Y0W8

Core Product Specifications and Parameters

Biological Background: SLC4A8 Function and Localization

• SLC4A8 encodes the electroneutral sodium bicarbonate exchanger 1, also known as electroneutral Na(+)-driven Cl-HCO3 exchanger or k-NBC3. It mediates electroneutral sodium- and carbonate-dependent chloride-bicarbonate exchange with a Na+:HCO3- stoichiometry of 2:1. Related references: PMID:18577713
• The protein plays a major role in pH regulation in neurons, contributing to acid-base homeostasis in the central nervous system.
• It is involved in sodium reabsorption in the renal cortical collecting ducts, supporting electrolyte balance.
• SLC4A8 is a multi-pass transmembrane protein localized to various cellular compartments, including apical and basolateral cell membranes, cytoplasmic vesicles, secretory vesicles, and synaptic vesicle membranes.
• The protein belongs to the solute carrier family 4 (SLC4) of bicarbonate transporters and undergoes alternative splicing, glycosylation, and disulfide bond formation.
• It is highly expressed in the brain (especially pyramidal cells of the hippocampus), spinal column, and testis, with moderate expression in trachea, thyroid, and kidney medulla, and lower levels in pancreas and kidney cortex. Expression is also detected in heart and kidney.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-90) of human SLC4A8, which may recognize multiple isoforms or splice variants.
• For Western blot, expect a band around 123kDa; slight shifts may occur due to post-translational modifications (e.g., glycosylation).
• Validate with brain or kidney lysates from human, mouse, or rat, and consider using the recombinant immunogen as a positive control in ELISA.
• Optimize antibody dilution and incubation conditions empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing for SLC4A8-related research in pH regulation, ion transport, and neuroscience. As a dedicated partner for diagnostic manufacturers, labs, and research institutes, we provide one-stop access to high-quality materials, technical services, and consulting from concept to clinic. Contact us to simplify your supply chain and accelerate assay development.