Anti-SLC4A5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BY07

Core Product Specifications and Parameters

Biological Background: SLC4A5 Function and Localization

• SLC4A5 encodes the electrogenic sodium bicarbonate cotransporter 4 (NBCe2), a member of the solute carrier 4 family.
• It mediates sodium- and bicarbonate-dependent electrogenic Na⁺ and HCO₃⁻ cotransport, with a stoichiometry varying from 1 Na⁺ to 2–3 HCO₃⁻.
• The protein localizes to both the apical and basolateral cell membranes, indicating a role in epithelial ion transport.
• Highest expression is observed in liver, spleen, and testis, with moderate levels in brain (choroid plexus, hippocampus, cerebrum, cerebellum) and kidney.
• It is also expressed in heart, pancreas, muscle, lung, placenta, stomach, small intestine, and peripheral blood leukocytes.
• The protein is predicted to contain multiple transmembrane helices, consistent with its transporter function.
• Alternative splicing generates several isoforms, potentially modulating transport activity.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight is approximately 126 kDa; consider using a 6–8% SDS‑PAGE gel for optimal resolution.
• Validate antibody reactivity in positive control tissues such as liver or kidney, where SLC4A5 is highly expressed.
• Immunohistochemistry (IHC‑P) may require antigen retrieval and permeabilization; confirm staining specificity with appropriate controls.
• Optimize antibody dilution for each application; a starting range of 1:500–1:2000 for WB and 1:50–1:200 for IHC‑P is commonly employed for polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high‑quality IVD raw materials, technical services, and consulting, supporting your entire development pipeline from concept to clinic. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. For ion transport studies, we offer this SLC4A5 polyclonal antibody along with sourcing solutions for related solute carrier family members.