Anti-SLC4A4 / NBC Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6R1

Core Product Specifications and Parameters

Biological Background: SLC4A4 / NBC Function and Localization

• SLC4A4, also known as NBCe1, kNBC1, or solute carrier family 4 member 4, is a multi-pass transmembrane glycoprotein and a member of the SLC4 bicarbonate transporter family.
• Functions as an electrogenic sodium/bicarbonate cotransporter with a Na+:HCO3- stoichiometry of 1:2 or 1:3, playing a critical role in bicarbonate reabsorption and intracellular pH regulation.
• Localizes predominantly to the basolateral membrane of polarized epithelial cells, including renal proximal tubule and pancreatic duct cells.
• Highly expressed in kidney proximal tubules and pancreas; also detected in corneal endothelium, heart, skeletal muscle, liver, salivary glands, prostate, colon, stomach, thyroid, brain, and spinal cord.
• Subject to alternative splicing, generating multiple isoforms (e.g., kNBC1, pNBC) with distinct N-terminal regions and tissue distributions.
• Post-translational modifications include phosphorylation and glycosylation, which may influence transporter activity and membrane targeting.
• Associated with human disease: mutations in SLC4A4 cause proximal renal tubular acidosis with ocular abnormalities and band keratopathy, and it is implicated in retinitis pigmentosa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 978-1078 of human SLC4A4 (NP_001091954.1), a region predicted to be intracellular; this design may enhance specificity for the NBCe1-A isoform.
• For Western blot (WB), a band around 121 kDa is expected; consider using appropriate positive controls such as kidney or pancreas lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), punctate basolateral membrane staining may be observed in polarized epithelial lines; validate staining with known localization markers.
• ELISA performance can be assessed using the immunizing peptide or recombinant protein fragments; cross-reactivity with mouse and rat orthologs should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider of IVD raw materials, supporting diagnostic developers with validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents. For researchers and manufacturers focused on renal physiology, acid-base homeostasis, or bicarbonate transport, this anti-SLC4A4 polyclonal antibody offers a reliable tool for target detection. Partner with CamelBio to streamline your raw material sourcing from early assay development to clinical application.