Anti-SLC4A2 Polyclonal Antibody for WB, ELISA - P04920

Core Product Specifications and Parameters

Biological Background: SLC4A2 Function and Localization

• Encoded by the SLC4A2 gene, anion exchange protein 2 (AE2) is a multi-pass membrane protein also known as non-erythroid band 3-like protein (BND3L) and solute carrier family 4 member 2.
• Functions as a sodium-independent anion exchanger, mediating the electroneutral exchange of chloride for bicarbonate ions across the plasma membrane, thereby regulating intracellular pH and cell volume. Related references: PMID:15184086 PMID:34668226
• Plays a critical role in osteoclast differentiation and function, regulating bone resorption and calpain-dependent actin cytoskeleton organization through anion exchange-dependent pH control. Related references: PMID:34668226
• Essential for intracellular pH regulation in CD8+ T-cells upon CD3 stimulation, modulating CD8+ T-cell responses.
• Localizes primarily to the basolateral and apical cell membranes, consistent with its role in epithelial ion transport.
• Expressed in the liver, stomach, kidney, prostate, thyroid, and rectum, with splice variants exhibiting tissue-specific distribution.
• Subject to post-translational modifications including methylation and palmitoylation, and exists in several alternatively spliced isoforms.
• Anion transport protein with structural features resolved via 3D-structure determination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200–300 of human SLC4A2 (NP_001186623.1), a region that may confer target specificity; consider sequence alignment to verify uniqueness across splice variants.
• For Western blotting, SLC4A2 is predicted at ~137kDa; use appropriate positive (e.g., HEK293, HepG2) and negative controls, and validate band identity with knockdown or knockout samples if available.
• In ELISA, optimize coating concentration and blocking conditions to minimize background; polyclonal antibodies may exhibit lot-to-lot variation, so titrate each new batch.
• Although polyclonal antibodies offer high sensitivity, confirm specificity in relevant biological matrices and consider cross-reactivity with other SLC4 family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, connecting diagnostic developers with validated antibody pairs, optimized monoclonal and polyclonal reagents, and bulk ancillary supplies. For projects centered on ion transport or osteoclast biology, we facilitate access to SLC4A2 antibodies, related family members, and rare-target reagents. Let CamelBio streamline your sourcing needs from concept to clinic.