Anti-SLC47A1 (MATE1) Rabbit Polyclonal Antibody for WB and ELISA - Q96FL8

Core Product Specifications and Parameters

Biological Background: SLC47A1 (MATE1) Function and Localization

• SLC47A1 (MATE1) is a multidrug and toxin extrusion protein belonging to the solute carrier family 47, functioning as an H⁺/organic cation antiporter. Related references: PMID:16330770, PMID:17509534

• It mediates the efflux of endogenous metabolites such as creatinine, thiamine (vitamin B1), agmatine, and estrone-3-sulfate, as well as various drugs and toxins, primarily in the kidney and liver. Related references: PMID:16330770, PMID:17495125, PMID:17509534

• The protein is localized to the apical cell membrane, consistent with its role in excretory transport into urine and bile.

• Tissue expression is widespread, with the highest levels in the adrenal gland and lower expression in the liver, skeletal muscle, and kidney; in the testis, it is found in seminiferous tubules, peritubular myoid cells, and Leydig cells, suggesting a role in the blood-testis barrier.

• Post-translational acetylation has been identified, and the gene undergoes alternative splicing, yielding multiple isoforms.

• A 3D structure is available, providing insights into the transport mechanism; the protein is part of the Reference proteome and has been identified through proteomics.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 460–550 of the C‑terminal region; the antibody is expected to recognize epitopes within this domain.
• For Western blot, a band of approximately 62 kDa is predicted; consider using lysates from human kidney (e.g., HEK293) or rat kidney/liver as positive controls, as these tissues express SLC47A1.
• In ELISA applications, coat the recombinant antigen or peptide fragment corresponding to the immunogen sequence and validate detection with appropriate secondary antibodies.
• Cross‑reactivity with human and rat is documented; verify performance in your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering a comprehensive portfolio from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. This anti‑SLC47A1 antibody supports research into drug transporters and pharmacokinetics; we can also assist in sourcing rare‑target antibodies or customized production to meet your diagnostic development needs.