Anti-SLC46A1 Rabbit Polyclonal Antibody for WB and ELISA - Q96NT5

Core Product Specifications and Parameters

Biological Background: SLC46A1 Function and Localization

• SLC46A1 encodes the proton-coupled folate transporter (PCFT/HCP1), a symporter that mediates cellular uptake of folates using a transmembrane H+ gradient as the driving force. Related references: PMID:17129779 PMID:17446347 PMID:17475902

• Transport occurs via alternating outward- and inward-open conformational states at acidic pH; protonation primes the protein, and folate binding triggers closure of the extracellular gate, leading to the inward-open state and release of substrates. Related references: PMID:32893190 PMID:34040256

• In addition to endogenous folates, PCFT transports antifolate chemotherapeutics such as methotrexate and pemetrexed, making it relevant to cancer and autoimmune disease research. Related references: PMID:18524888 PMID:19762432 PMID:22345511

• An alternative splicing isoform exists that is unable to mediate proton-coupled folate transport.

• SLC46A1 also functions as a lower-affinity, pH-independent heme carrier, constituting a major intestinal heme importer and participating in heme trafficking in retina, brain, liver, and kidney. Related references: PMID:17156779 PMID:32621820

• Subcellular localization includes the cell membrane, apical and basolateral membranes, endosome membrane, and cytoplasm.

• Expression is highest in the upper small intestine (duodenum and jejunum), with lower levels in kidney, liver, placenta, and retina; very low in brain, lung, heart, and muscle. Related references: PMID:17129779 PMID:19762432 PMID:17335806

• Post-translational modifications include acetylation, disulfide bond formation, glycosylation, and phosphorylation, which may regulate transporter function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 130 amino acids of human SLC46A1; this region may be accessible in native and denatured conformations, supporting WB and ELISA.
• For WB, consider using protein lysates from tissues with high SLC46A1 expression (e.g., small intestine, kidney, liver). The predicted molecular weight is ~50 kDa; note that glycosylation can cause band shifts.
• Verify specificity by comparing signals in SLC46A1‑knockdown or knockout samples, or by using blocking peptide competition.
• For ELISA, coat with recombinant SLC46A1 protein or peptide encompassing the immunogen sequence; optimize coating buffer and blocking conditions.
• The antibody cross-reacts with mouse and rat SLC46A1, making it suitable for studies in rodent models of folate or heme transport.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. This anti-SLC46A1 polyclonal antibody supports research into folate transport, heme trafficking, and antifolate drug uptake. For rare or custom targets, our one-stop sourcing bridges supply gaps from concept to clinic.