Anti-SLC40A1 Rabbit Polyclonal Antibody for WB, ELISA - Q9NP59

Core Product Specifications and Parameters

Biological Background: SLC40A1 (Ferroportin) Function and Localization

• Also known as ferroportin-1, iron-regulated transporter 1, and solute carrier family 40 member 1 (SLC40A1), this protein is the sole known mammalian iron efflux transporter.
• Transports Fe(2+) across the plasma membrane from the cytosol to the extracellular space, essential for dietary iron absorption in enterocytes, iron recycling from macrophages, and iron mobilization from hepatocytes.
  Related references: PMID:15692071 PMID:22178646 PMID:22682227
• The export activity is negatively regulated by the hormone hepcidin (HAMP); hepcidin binding triggers SLC40A1 internalization and ubiquitin-dependent degradation, thereby decreasing serum iron levels.
  Related references: PMID:22682227 PMID:29237594 PMID:32814342
• Localized to the plasma membrane, with basolateral distribution in polarized epithelial cells such as enterocytes.
• Expressed in key iron-handling tissues: placenta, intestine, muscle, spleen, and highly expressed in erythrocytes and mature red blood cells (at protein level).
  Related references: PMID:10747949 PMID:23219802 PMID:29599243
• Post-translationally modified; it is a glycoprotein and may be ubiquitinated, with mutations linked to hemochromatosis type 4 (disease variant).

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to residues 200–270 of human SLC40A1; antigen design suggests potential recognition of endogenous ferroportin.
• For Western blot, the expected molecular weight is approximately 63 kDa; include appropriate positive controls (e.g., duodenum or liver lysates) and note that glycosylation may cause size shifts.
• Cross-reactivity with human and mouse is reported; validate species-specific reactivity in your assay if using mouse samples.
• For ELISA applications, consider indirect or sandwich formats; optimize coating concentration and secondary antibody dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for diagnostic raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. Our expertise in iron metabolism targets like SLC40A1 supports your assay development from concept to clinic. Contact us for bulk orders and customized sourcing needs.