Anti-SLC3A2/CD98hc Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P08195

Core Product Specifications and Parameters

Biological Background: SLC3A2/CD98hc Function and Localization

• SLC3A2, also known as 4F2 heavy chain or CD98hc, is a single-pass type II membrane protein that serves as the heavy chain subunit of amino acid transporters. It forms heterodimers with various SLC7 family light chains to facilitate amino acid transport across the plasma membrane. Related references: PMID:10574970, PMID:10903140, PMID:11557028
• It functions as a chaperone, essential for the biogenesis, trafficking, and plasma membrane localization of the heterodimeric transporter complexes, such as SLC7A5/LAT1 and SLC7A8/LAT2. Related references: PMID:10391915, PMID:10574970, PMID:11311135
• Heterodimers with SLC7A5 or SLC7A8 mediate the sodium-independent uptake of large neutral amino acids, thyroid hormones, and L-DOPA. Related references: PMID:10574970, PMID:11389679, PMID:11557028
• SLC3A2/SLC7A11 functions as a cystine/glutamate antiporter, exchanging extracellular cystine for intracellular glutamate, which is critical for glutathione synthesis and oxidative stress response. Related references: PMID:34880232
• Beyond transport, SLC3A2 modulates integrin-dependent cell adhesion, spreading, migration, and tumor progression through its cytoplasmic domain. Related references: PMID:11121428, PMID:15625115
• Subcellularly, SLC3A2 localizes to the plasma membrane (both apical and basolateral sides), cell junctions, lysosomes, and melanosomes, reflecting its diverse trafficking roles.
• The protein undergoes multiple post-translational modifications including glycosylation, disulfide bond formation, phosphorylation, and acetylation, which regulate its stability and function.
• In the context of infection, SLC3A2 serves as a co-receptor for hepatitis C virus (HCV) entry and facilitates malaria parasite Plasmodium vivax invasion of immature red blood cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 206-630) of human SLC3A2, suggesting the antibody may recognize the native protein in cell surface and secreted forms.
• Western blot (WB) applications should consider using reducing conditions to resolve the predicted protein weight of 58 kDa, and validate in cell lines or tissues with known SLC3A2 expression (e.g., kidney, placenta).
• For immunohistochemistry (IHC-P), preliminary testing on paraffin-embedded tissue sections with appropriate antigen retrieval is advised; include known positive and negative controls for specificity.
• Given the polyclonal nature, lot-to-lot consistency should be monitored in quantitative assays, and titration experiments are recommended for optimal dilution.
• Cross-reactivity with mouse and rat orthologs is reported, enabling translational studies, but sequence alignment of the immunogen region is advisable for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted provider of IVD raw materials, offering a streamlined sourcing experience for diagnostic manufacturers and research laboratories. This anti-SLC3A2/CD98hc polyclonal antibody exemplifies our commitment to supplying high-quality reagents for amino acid transport and cancer biology research. In addition to antibodies, we support customers with ancillary reagents, custom antibody development, and sourcing of rare targets to accelerate assay development from concept to clinic.