Anti-SLC3A2/CD98hc Rabbit Polyclonal Antibody for WB, ELISA - P08195

Core Product Specifications and Parameters

Biological Background: SLC3A2/CD98hc Function and Localization

• SLC3A2 (CD98 heavy chain) is a type II transmembrane glycoprotein that serves as a chaperone for the biogenesis and trafficking of functional amino acid transporter heterodimers to the plasma membrane. It associates with various SLC7 family light chains (e.g., SLC7A5/LAT1, SLC7A8/LAT2, SLC7A11/xCT) to form amino acid antiporters with distinct substrate specificities. Related references: PMID:10574970, PMID:10903140, PMID:11557028, PMID:10391915, PMID:11311135

• The SLC3A2/SLC7A5 heterodimer mediates the uptake of large neutral amino acids, thyroid hormones, and L-DOPA, and is also involved in mTORC1 activation upon leucine transport into lysosomes when associated with LAPTM4B. Related references: PMID:10574970, PMID:11389679, PMID:11557028, PMID:25998567

• SLC3A2/SLC7A11 functions as a cystine/glutamate antiporter critical for glutathione synthesis and redox balance. Related references: PMID:34880232

• SLC3A2 modulates integrin signaling and is essential for integrin-dependent cell spreading, migration, and tumor progression. Related references: PMID:11121428, PMID:15625115

• During viral infection, SLC3A2/SLC7A5 facilitates hepatitis C virus (HCV) entry and enhances mTORC1 signaling to promote viral propagation. It also acts as a receptor for Plasmodium vivax malaria parasites in reticulocytes.

• SLC3A2 is expressed at the cell membrane (apical and basolateral), cell junctions, lysosomes, and melanosomes, consistent with its role in amino acid transport and signaling.

• Ubiquitously expressed with highest levels in kidney, placenta, and testis; also detected in endothelial cells, T-lymphocytes, and various cancer cells.

• Post-translational modifications include N-glycosylation, phosphorylation, acetylation, disulfide bond formation, and ubiquitination, which may regulate its stability and interactions.

Experimental Guidance and Technical Tips

• For Western blotting, prepare whole-cell lysates from human, mouse, or rat cell lines expressing SLC3A2. The expected molecular weight is approximately 58 kDa, though glycosylation may produce higher molecular weight bands. Use appropriate reducing conditions for SDS-PAGE.

• In ELISA applications, consider using the recombinant protein immunogen (amino acids 461-630) as a positive control to validate antibody reactivity. Perform titration experiments to optimize coating and detection antibody concentrations.

• Although this antibody cross-reacts with mouse and rat, validation in the specific tissue or cellular context is recommended, as expression levels and post-translational modifications can vary.

• For immunohistochemistry or immunoprecipitation, extensive optimization may be required; these applications are not listed in the standard specifications, so perform pilot experiments to assess suitability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes by providing high-quality IVD raw materials such as validated antibody pairs, polyclonal antibodies, and recombinant proteins for SLC3A2 and related transporter targets. Our one-stop sourcing platform enables streamlined procurement of bulk reagents, rare target antibodies, and technical consultation to accelerate your assay development from concept to clinic.