Anti-SLC3A1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q07837

Core Product Specifications and Parameters

Biological Background: SLC3A1 Function and Localization

• SLC3A1, also known as rBAT (related to b(0,+) amino acid transporter), is the heavy chain subunit that chaperones light chain catalytic subunits to the plasma membrane for functional amino acid transport.
• It forms a heteromeric complex with SLC7A9, mediating electrogenic exchange of cationic amino acids and neutral amino acids with high affinity for L-cystine and dibasic amino acids, playing a crucial role in renal and intestinal reabsorption (PubMed:10588648, PubMed:11318953, PubMed:16609684). Related references: PMID:10588648, PMID:11318953, PMID:16609684
• SLC3A1 also partners with SLC7A13 to form a functional transporter for anionic and neutral amino acids, likely contributing to L-cystine reabsorption in late proximal tubules (By similarity).
• The protein is a type II membrane glycoprotein with an N-terminal signal-anchor, a single transmembrane helix, and a large extracellular domain.
• It is predominantly expressed at the brush border membrane of kidney proximal tubules, small intestine, and pancreas, with weaker expression in the liver.
• Genetic mutations in SLC3A1 are associated with cystinuria type I, an inherited disorder of dibasic amino acid transport, underscoring its clinical significance.
• Post-translational modifications include N-linked glycosylation and phosphorylation, which may regulate its stability and interaction with light chains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal extracellular region (aa 416-685) of human SLC3A1; this region is well-conserved across human, mouse, and rat, supporting the declared cross-reactivity.
• For Western blotting, target an apparent molecular weight of ~79 kDa; consider using reducing conditions and validate in kidney or intestinal tissue lysates where expression is highest.
• In immunohistochemistry (IHC-P), antigen retrieval may be required; test in formalin-fixed paraffin-embedded kidney sections to confirm apical membrane staining.
• For ELISA applications, this antibody can be used as capture or detection reagent; pairing with an SLC7A9-specific antibody may enable detection of the functional heteromer.
• Perform initial optimization experiments in samples from human, mouse, or rat origin to confirm species cross-reactivity and avoid nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material sourcing, offering a comprehensive catalog of antibodies and ancillary reagents to accelerate diagnostic development. The SLC3A1 rabbit polyclonal antibody is part of our transporter protein portfolio, enabling research into amino acid transport disorders such as cystinuria. Beyond individual antibodies, we supply validated antibody pairs, bulk quantities, and custom development services to support your entire assay workflow—from concept to clinical application.