Anti-SLC39A4 Polyclonal Antibody for WB, IHC-P, ELISA - Q6P5W5

Core Product Specifications and Parameters

Biological Background: SLC39A4 (ZIP4) Function and Localization

• SLC39A4 encodes the zinc transporter ZIP4, a member of the solute carrier family 39 and also designated Zrt- and Irt-like protein 4 (ZIP-4).
• It functions as a selective Zn²⁺ uniporter (PubMed:17202136, PubMed:22242765, PubMed:27321477) and is essential for dietary zinc absorption across the intestinal epithelium (By similarity). Related references: PMID:17202136, PMID:22242765, PMID:27321477
• The transporter exhibits polyspecific binding and can mediate uptake of Cu²⁺, Cd²⁺, and possibly Ni²⁺ at elevated concentrations (PubMed:22242765, PubMed:31914589). Related references: PMID:22242765, PMID:31914589
• Localizes to the cell membrane, recycling endosome membrane, and apical cell membrane, consistent with roles in zinc trafficking and endosomal recycling.
• Highly expressed in the small intestine (jejunum and duodenum), stomach, colon, and kidney, reflecting its critical function in dietary zinc uptake.
• Post-translational modifications include disulfide bond formation, glycosylation, and ubiquitin conjugation; alternative splicing yields multiple isoforms.
• Classified within transmembrane transport and metal-binding pathways, featuring a signal peptide, multiple transmembrane helices, and metal-ion coordination sites.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular region (aa 23–327), which may capture both full-length and processed forms of ZIP4; confirm detection of the predicted ~68 kDa band in WB, noting possible shifts from glycosylation.
• For IHC-P, use human kidney or small intestine sections as a positive control due to strong ZIP4 expression; antigen retrieval conditions may need optimization.
• ELISA assays can be developed using recombinant ZIP4 protein or peptide coating; consider a sandwich format with a matched detection antibody if high specificity is required.
• As a polyclonal antibody, expect some lot-to-lot variability; titrate working dilutions for each application and validate in the relevant species (human, mouse, rat).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a seamless supply of high-quality IVD raw materials, including the anti-SLC39A4 polyclonal antibody for zinc transporter studies. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal alternatives, and bulk ancillary reagents to support your assay development. From concept to clinic, trust CamelBio as your strategic partner for rare target sourcing and comprehensive technical solutions.