Anti-SLC35D3 Polyclonal Antibody for WB, ELISA - Q5M8T2

Core Product Specifications and Parameters

Biological Background: SLC35D3 Function and Localization

• SLC35D3 (Solute carrier family 35 member D3), also known as Fringe connection-like protein 1 (FRCL1), is a multi-pass transmembrane protein belonging to the nucleotide-sugar transporter family.
• It functions as a probable UDP-glucose transporter, mediating the transport of UDP-glucose from the cytosol into the lumen of synaptic vesicles [PMID:34269178]. Related references: PMID:34269178
• SLC35D3 may also act as a molecular adapter promoting the formation of the PI3KC3-C1 autophagy initiation complex to enhance autophagy in dopaminergic neurons.
• It has been reported to regulate the plasma membrane localization of the D(1A) dopamine receptor (DRD1) and modulate dopamine signaling.
• SLC35D3 localizes primarily to the membranes of synaptic vesicles, early endosomes, and the endoplasmic reticulum, consistent with roles in vesicular trafficking and secretion.
• By similarity, SLC35D3 is involved in the maturation of platelet dense granules, suggesting broader roles in organelle biogenesis.
• Key structural features include multiple transmembrane helices, and its function is associated with sugar transport across intracellular membranes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 310–390 of human SLC35D3, corresponding to a C‑terminal cytoplasmic region. This design may favor detection of full‑length protein and potential C‑terminal variants.
• For Western blotting, the predicted molecular weight is approximately 44 kDa. Consider using neuronal or brain tissue lysates as positive controls, given SLC35D3's expression in synaptic vesicles and dopaminergic neurons.
• In ELISA applications, this polyclonal antibody can be used for antigen capture or detection; optimization of coating conditions and antibody titration is recommended.
• Cross‑reactivity is confirmed for human SLC35D3; due to sequence conservation, reactivity with other species may be possible but should be experimentally validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high‑quality IVD raw materials. This SLC35D3 polyclonal antibody supports studies in synaptic biology and neurodegeneration. For your IVD development needs, we offer validated antibody pairs, bulk reagents, and custom sourcing for rare or specialized targets.