Anti-SLC35A2 Polyclonal Antibody for IF/ICC and ELISA - P78381

Core Product Specifications and Parameters

Biological Background: SLC35A2 Function and Localization

• SLC35A2, also known as UDP-galactose translocator (UGT), is a member of the solute carrier family 35 (SLC35) that mediates the transport of nucleotide sugars across the Golgi membrane.
• It functions as an antiporter, exchanging cytosolic UDP-galactose for UMP (and to a lesser extent AMP and CMP) into the Golgi lumen, providing substrate for galactosyltransferases. Related references: PMID:12682060, PMID:9010752
• SLC35A2 also transports UDP-N-acetylgalactosamine (UDP-GalNAc) and other nucleotide sugars, indicating broad substrate specificity. Related references: PMID:11784306, PMID:12682060
• The translocator is essential for glycosphingolipid biosynthesis; it is required for globotriaosylceramide (Gb3Cer) synthesis from lactosylceramide, linking it to the Pk blood group system. Related references: PMID:30817854
• Subcellularly, SLC35A2 localizes primarily to the Golgi apparatus membrane, with additional presence in the endoplasmic reticulum membrane, consistent with its role in secretory pathway glycosylation.
• Mutations in SLC35A2 are associated with congenital disorder of glycosylation type IIm (CDG2M) and early infantile epileptic encephalopathy, highlighting its clinical significance.
• The protein is predicted to contain multiple transmembrane helices and undergoes alternative splicing, producing different isoforms.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 1-242 of human SLC35A2, covering the N-terminal region. This region includes the likely cytosolically oriented domain and some transmembrane segments, which may contribute to antibody reactivity in various applications.
• For immunofluorescence (IF/ICC), consider using permeabilization to detect the intracellular Golgi localization. A Golgi marker co-stain is recommended to confirm specificity.
• In ELISA, this antibody can be used as a primary detection reagent; however, optimal dilutions and assay conditions should be determined empirically in the user's experimental system.
• Cross-reactivity has been validated only for human samples. When working with other species, sequence homology should be assessed, and cross-reactivity should be experimentally verified.
• The protein has a predicted molecular weight of 41 kDa; in Western blot (not listed as an application), if attempted, a band around this size may be observed, but post-translational modifications could affect migration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This anti-SLC35A2 polyclonal antibody supports research into glycosylation disorders and Golgi biology. Whether you need bulk quantities, customized conjugations, or technical consultation, CamelBio streamlines your sourcing from discovery to diagnostic development.