Anti-SLC35A1 Rabbit Polyclonal Antibody for WB, ELISA - P78382

Core Product Specifications and Parameters

Biological Background: SLC35A1 Function and Localization

• SLC35A1 encodes a CMP-sialic acid transporter (solute carrier family 35 member A1) belonging to the nucleotide sugar transporter family SLC35.
• The protein localizes to the Golgi apparatus membrane, where it functions as an antiporter exchanging cytosolic CMP-sialic acid for luminal CMP, and can also exchange CMP-sialic acid for AMP or UMP. Related references: PMID:12682060 PMID:15576474 PMID:23873973
• The transporter shows higher binding affinity for free CMP than for CMP-sialic acid and also mediates CDP-ribitol transport (By similarity).
• Mutations in SLC35A1 are associated with congenital disorder of glycosylation type IIf (CDG2F), a condition characterized by defective protein glycosylation.
• The gene undergoes alternative splicing, potentially producing multiple protein isoforms (UniProtKB keyword).
• Additional features include multiple transmembrane domains and a role in sugar transport as an antiporter.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-100) of human SLC35A1, which may contribute to specific target recognition.
• For Western blot, expect a band of approximately 37 kDa; consider using HeLa or HEK293 cell lysates as positive controls and optimize antibody dilution.
• In ELISA, the antibody can be applied for direct or indirect detection; account for cross-reactivity with mouse samples when designing experiments.
• As with any polyclonal antibody, verify lot-to-lot consistency and include appropriate blocking steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting research and diagnostic development from concept to clinic. This anti-SLC35A1 antibody is applicable to glycosylation and Golgi transport studies. We provide validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials to accelerate your assay development.