Anti-SLC34A3 Rabbit Polyclonal Antibody for WB/ELISA - Q8N130

Core Product Specifications and Parameters

Biological Background: SLC34A3 Function and Localization

• SLC34A3 encodes the sodium-dependent phosphate transport protein 2C (NaPi-2c), also known as Na(+)/Pi cotransporter 2C, a member of the solute carrier family 34 (SLC34) that mediates cellular phosphate uptake.
• The transporter actively cotransports phosphate with sodium ions at a 2:1 Na⁺:Pi stoichiometry, functioning as an electroneutral symporter in the renal brush border membrane.
  Related references: PMID:11880379
• In humans, NaPi-2c is predominantly expressed in the kidney, localized to the apical membrane of proximal tubular epithelial cells, where it plays a critical role in phosphate homeostasis.
• Mutations in SLC34A3 are linked to hereditary hypophosphatemic rickets with hypercalciuria (HHRH), highlighting its clinical importance in renal phosphate wasting disorders.
• The protein undergoes glycosylation and phosphorylation and contains structural features such as disulfide bonds and multiple transmembrane helices, with a molecular weight of approximately 64 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–117) of human SLC34A3; this antibody may recognize both full-length protein and N-terminal fragments in denaturing Western blots.
• For Western blotting, use kidney tissue lysates or SLC34A3-transfected cell lysates; expect a specific band around 64 kDa. Consider using reducing SDS-PAGE conditions to resolve disulfide-linked complexes.
• In ELISA, coat with recombinant SLC34A3 protein or a peptide encompassing the immunogen sequence, and titer the antibody to determine optimal working concentrations for your specific assay format.
• Cross-reactivity with mouse and rat SLC34A3 has been reported; validate the antibody in the relevant sample system, as detection may vary depending on sequence homology in the N-terminal region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering diagnostic manufacturers and labs access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This SLC34A3 polyclonal antibody supports research in renal phosphate transport and kidney physiology, and is part of our portfolio of cell membrane transporter targets. Our global supply network can also assist in procuring rare or custom raw materials, streamlining your development pathway from concept to clinic.