Anti-SLC34A1 Rabbit Polyclonal Antibody for WB and ELISA - Q06495

Core Product Specifications and Parameters

Biological Background: Sodium-Dependent Phosphate Transport Protein 2A Function and Localization

• SLC34A1 encodes the sodium-dependent phosphate transport protein 2A (NaPi-2a), a member of the solute carrier family 34.
• It mediates active, electrogenic phosphate uptake into renal proximal tubule cells via Na+ cotransport with a 3:1 Na+:Pi stoichiometry. Related references: PMID:12324554, PMID:20335586, PMID:26047794
• The protein localizes to the apical brush border membrane of kidney epithelial cells, consistent with its role in phosphate reabsorption.
• Tissue expression is primarily observed in kidney and lung.
• Post-translational modifications include glycosylation and phosphorylation, and the protein contains disulfide bonds.
• Alternative splicing produces multiple isoforms, potentially modulating transport activity or localization.
• Mutations in SLC34A1 are associated with disease variants, including osteoporosis, highlighting its physiological importance.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 562–639), which is predicted to be intracellular. Consider this when designing membrane protein extraction and denaturation protocols.
• For WB, a band near 69 kDa is expected, though glycosylation may cause a higher apparent molecular weight. Validate expression using kidney tissue or cell lines.
• Due to homology among species (human, mouse, rat), this antibody may be suitable for cross-species studies; confirm reactivity in the relevant sample system.
• For ELISA, the recombinant immunogen or target-derived peptides can be used as coating antigens; optimize coating concentration and blocking conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk reagents, and rare target sourcing. This anti-SLC34A1 polyclonal antibody, targeting the renal phosphate transporter, complements our offerings for kidney disease and metabolic disorder research. Contact us for custom protein expression, conjugation, or large-scale production needs.