Anti-SLC32A1/VGAT Polyclonal Antibody for WB and ELISA - Q9H598

Core Product Specifications and Parameters

Biological Background: SLC32A1/VGAT Function and Localization

• SLC32A1 encodes the vesicular inhibitory amino acid transporter (VIAAT/VGAT), which mediates the uptake of the inhibitory neurotransmitters GABA and glycine into synaptic vesicles.
• The transporter functions as an antiporter, exchanging intravesicular protons for cytosolic 4-aminobutanoate (GABA) or, to a lesser extent, glycine; this process relies on both the chemical and electrical components of the proton gradient.
• Localized primarily to the presynaptic membrane of cytoplasmic vesicles in GABAergic and glycinergic neurons, ensuring proper packaging of inhibitory neurotransmitters.
• Highly expressed in the retina, particularly in horizontal cell terminals, consistent with its role in visual processing and lateral inhibition.
• Post-translational modifications include nitration, which may influence transporter activity or stability under conditions of oxidative stress.
• Clinically, variants in SLC32A1 have been associated with epilepsy and intellectual disability, highlighting the critical importance of inhibitory neurotransmission.
• As a member of the solute carrier family 32, the protein contains ten predicted transmembrane helices and is conserved across human, mouse, and rat.
• The mature protein has an experimentally determined molecular weight of approximately 57 kDa, consistent with its UniProt-calculated mass of 57,415 Da.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–120) of human SLC32A1, which is predicted to be intracellular. Consider appropriate permeabilization steps if adapting this antibody for ICC or IHC, and note that it has been validated only for WB and ELISA.
• For Western blotting, expect a principal band near 57 kDa; include proper loading controls and confirm cross-reactivity when using mouse or rat samples.
• As a polyclonal antibody, lot-to-lot variability may occur; validate each new batch for consistent performance in your specific assay system.
• In ELISA applications, optimize coating and detection conditions, using the recombinant immunogen as a positive control and reference standard.
• Due to its cross-reactivity with human, mouse, and rat, this antibody is well-suited for comparative studies across species in inhibitory synapse research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers, offering a one-stop source for IVD raw materials, including this anti-SLC32A1/VGAT polyclonal antibody targeting inhibitory neurotransmitter pathways. We support your development pipeline with validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and specialized sourcing for rare or emerging targets to accelerate your assay development from concept to clinic.