Anti-SLC32A1 Rabbit Polyclonal Antibody for WB - Q9H598

Core Product Specifications and Parameters

Biological Background: SLC32A1 Function and Localization

• SLC32A1 encodes vesicular inhibitory amino acid transporter (VGAT/VIAAT), a member of the solute carrier family 32.
• Functions as a proton-coupled antiporter that loads inhibitory neurotransmitters GABA and glycine into synaptic vesicles, enabling their release at nerve terminals (By similarity).
• Efficient transport depends on both chemical (ΔpH) and electrical (Δψ) components of the proton gradient across the vesicle membrane (By similarity).
• Localizes to the membrane of synaptic vesicles at presynaptic terminals, specifically in GABAergic and glycinergic neurons.
• Highly expressed in the retina, particularly in horizontal cell terminals, suggesting a role in inhibitory signaling in the visual system.
• Post-translational modifications include nitration, which may regulate transporter activity under physiological or pathological conditions.
• Genetic variants in SLC32A1 have been associated with neurological disorders, including epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The full-length immunogen (aa 1-525) is expected to elicit polyclonal antibodies recognizing multiple epitopes, enhancing sensitivity in WB and ELISA.
• For WB, consider using reducing SDS-PAGE to detect the ~57 kDa band; validate the antibody in relevant brain or retinal tissue lysates from human, mouse, or rat.
• In ELISA, the antibody may be used as a capture or detection reagent after appropriate titration and buffer optimization.
• Cross-reactivity with mouse and rat SLC32A1 is anticipated due to high sequence homology; however, perform species-specific validation in the intended sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research labs by providing one-stop access to critical reagents like this SLC32A1 polyclonal antibody. Our expertise covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, all tailored for neurotransmitter and synaptic vesicle research. Partner with CamelBio to bridge your raw-material needs from discovery to clinical application with efficiency and reliability.