Anti-SLC31A1 Polyclonal Antibody for WB, ELISA - O15431

Core Product Specifications and Parameters

Biological Background: SLC31A1 Function and Localization

• SLC31A1, also known as CTR1 or COPT1, is a high-affinity copper transporter belonging to the solute carrier family 31. It facilitates the uniport of copper(1+) across the plasma membrane into the cytoplasm. Related references: PMID:11734551, PMID:16135512, PMID:17525160
• The protein directly delivers copper(1+) to specific chaperones such as ATOX1 via a transient interaction with its C-terminal domain, playing a critical role in intracellular copper homeostasis. Related references: PMID:11734551, PMID:16135512, PMID:17525160
• SLC31A1 also mediates uptake of platinum-containing chemotherapeutic agents (e.g., cisplatin) and other metals like silver(1+) and cadmium(2+), underscoring its pharmacological relevance. Related references: PMID:20451502, PMID:20569931, PMID:33294387
• Functioning as a redox sensor, the protein promotes angiogenesis via VEGF-induced ROS signaling through sulfenylation at Cys-189 and disulfide bond formation with KDR, independent of its transport activity. Related references: PMID:35027734
• Subcellularly, it localizes to the plasma membrane and various endosomal membranes (early, recycling, late) as well as apical and basolateral membranes in polarized cells, facilitating copper mobilization.
• The protein is implicated in neurodegeneration and epilepsy according to UniProt keywords, with documented roles in copper metabolic diseases.
• Post-translational modifications include disulfide bonds, glycosylation, and phosphorylation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-60 amino acids of human SLC31A1, a region likely involved in copper(2+) reduction and metal binding; this design may enable detection of both unmodified and early post-translationally modified forms.
• For western blotting, expect a predominant band at approximately 21 kDa under reducing conditions; however, note that potential glycosylation or dimerization might yield higher molecular weight species, so consider extended blocking and optimized lysis buffers.
• Given the antibody's reactivity with human and mouse SLC31A1, validate specificity in relevant cell lines or tissues known to express the transporter (e.g., intestinal epithelial cells, hepatocytes, or endothelial cells).
• For ELISA applications, pairing with a detection antibody targeting a distant epitope could improve sensitivity; however, titration and validation in the specific sample matrix are recommended.
• As SLC31A1 is a membrane protein, use mild detergents during sample preparation to preserve antigenicity and avoid harsh reducing conditions that might disrupt conformational epitopes recognized by this polyclonal antibody.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we support diagnostic manufacturers and researchers with reliable access to high-quality IVD raw materials like this anti-SLC31A1 antibody. Our extensive portfolio includes validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents for immunoassay development. Whether you need copper metabolism markers or rare target sourcing, our technical and supply chain expertise bridges your project from concept to clinic.