Anti-SLC30A1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y6M5

Core Product Specifications and Parameters

Biological Background: SLC30A1 Function and Localization

• SLC30A1 (Zinc transporter 1, ZnT-1) is a proton-coupled zinc antiporter that mediates zinc efflux from cells, protecting against intracellular zinc accumulation and toxicity. Related references: PMID:31471319, PMID:33996761, PMID:39422023
• It also prevents the transport of CACNB2 to the plasma membrane, thereby modulating L-type calcium channel expression and inhibiting calcium and heavy metal influx (By similarity).
• In macrophages, the antiporter increases zinc concentration in cytoplasmic vesicles containing engulfed bacteria, contributing to pathogen inactivation. Related references: PMID:32441444
• SLC30A1 forms a complex with TMC6/EVER1 and TMC8/EVER2 at the ER membrane in keratinocytes, facilitating zinc uptake into the ER and downregulating transcription factors induced by zinc and cytokines. Related references: PMID:18158319
• Additional functions include copper transport mediating cuproptosis and acting as a Zn²⁺/Ca²⁺ exchanger exporting zinc in exchange for calcium influx. Related references: PMID:39111308, PMID:38669333
• Subcellular localization extends to the plasma membrane (basolateral), cytoplasmic vesicle membranes, endoplasmic reticulum, Golgi apparatus, nucleus, and postsynaptic density, consistent with multifaceted ion transport roles.
• The protein is phosphorylated and glycosylated, and contains transmembrane helices and metal-binding domains as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 328–507 in the C-terminal region of human SLC30A1; consider this when interpreting band specificity in WB.
• For IHC-P, optimize antigen retrieval conditions and validate staining in relevant human, mouse, or rat tissues.
• Perform appropriate negative controls (e.g., peptide blocking or knockout lysates) to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, empowering researchers studying zinc homeostasis and metal ion transport. We supply validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents for immunoassays, and specialize in sourcing rare-target antibodies like anti-SLC30A1 to accelerate your diagnostic development.