Anti-SLC2A4RG Polyclonal Antibody for WB, ELISA - Q9NR83

Core Product Specifications and Parameters

Biological Background: SLC2A4RG Function and Localization

• SLC2A4RG, also known as GLUT4 enhancer factor (GEF) or HDBP-1, is a transcription factor that regulates SLC2A4 (GLUT4) and Huntington disease (HD) gene expression.
• It binds to the consensus DNA sequence 5'-GCCGGCG-3' to transactivate target genes involved in glucose metabolism and neurodegeneration.
• The protein localizes to both the cytoplasm and nucleus, shuttling possibly in response to cellular signals.
• Tissue expression profiling reveals expression in heart, skeletal muscle, liver, kidney, and pancreas, with low or undetectable levels in lung, placenta, and brain; another study reports ubiquitous expression with minimal levels in brain and ileum.
• Keywords indicate it is a zinc-finger DNA-binding phosphoprotein, suggesting regulation via post-translational modifications and potential metal-ion dependency.
• Alternative splicing of the SLC2A4RG gene produces multiple isoforms, which may exhibit tissue-specific functions.

Experimental Guidance and Technical Tips

• The immunogen, a synthetic peptide within amino acids 100-200 of human SLC2A4RG, targets an internal region; consider using this antibody for detecting both endogenous and overexpressed SLC2A4RG in Western blotting.
• For ELISA applications, verify the antibody's performance with your specific antigen coating conditions and detection system, as peptide-based immunogens may yield varying reactivity.
• Given the predicted molecular weight of ~41 kDa, ensure proper gel percentage and transfer conditions for optimal resolution; validate the band size in your experimental samples.
• Cross-reactivity with mouse is reported; if working with mouse tissues, confirm specificity via knockdown or knockout controls to account for potential off-target binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated sourcing solutions for your diagnostic development needs. For transcription factor targets like SLC2A4RG, we supply validated polyclonal antibodies, monoclonal pairs, and ancillary reagents to accelerate your assay development. Contact us to leverage our global network for rare target antibodies and bulk raw materials.