Anti-SLC29A3 Rabbit Polyclonal Antibody for WB, ELISA - Q9BZD2

Core Product Specifications and Parameters

Biological Background: SLC29A3 Function and Localization

• SLC29A3 encodes equilibrative nucleoside transporter 3 (ENT3), a multi-pass membrane protein belonging to the solute carrier 29 family.
• It functions as a Na+-independent, facilitative uniporter for a broad range of nucleosides (adenosine, guanosine, uridine, thymidine, cytidine, inosine) and nucleobases, with transport activity enhanced at acidic pH. Related references: PMID:15701636, PMID:19164483, PMID:20595384, PMID:28729424
• Mediates export of nucleosides from lysosomes to cytosol and import into mitochondria, thereby facilitating nucleic acid salvage pathways. Related references: PMID:28729424
• Additionally capable of transporting monoamine neurotransmitters (dopamine, serotonin, noradrenaline, tyramine) and ATP. Related references: PMID:19164483
• Regulates macrophage function via lysosomal nucleoside export (By similarity).
• Acts as a lysosomal urate exporter and modulates inflammasome activation through its dual role in adenosine and urate transport. Related references: PMID:41476938
• Subcellularly localizes to lysosome membrane, late endosome membrane, mitochondrion membrane, and cell membrane.
• Widely expressed in adult and fetal tissues, with highest levels in placenta, uterus, ovary, spleen, lymph node, bone marrow, and liver; lower levels in brain and heart; also expressed in macrophages. Related references: PMID:15701636, PMID:19164483, PMID:22174130

Experimental Guidance and Technical Tips

• The immunogen spans the N‑terminal region (amino acids 1–110), which may be exposed in certain conformations; consider this when designing detection protocols for native versus denatured protein.
• For WB, the predicted molecular weight is approximately 52 kDa; validate with positive control lysates from tissues known to express high levels of SLC29A3, such as placenta or spleen.
• For ELISA, optimize coating conditions and confirm reactivity with recombinant or endogenous SLC29A3 in your sample system.
• Given the reported localization to lysosomes and mitochondria, subcellular fractionation or organelle enrichment may improve detection sensitivity in complex samples.
• Cross‑reactivity is reported for human; if working with other species, experimental validation is recommended.

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CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-SLC29A3 pAb. Beyond antibodies, we supply bulk ancillary reagents and assist in sourcing rare-target raw materials, supporting your nucleoside transport and immunology research applications from concept to clinic.