Anti-SLC29A1 Rabbit Polyclonal Antibody for WB, ELISA - Q99808

Core Product Specifications and Parameters

Biological Background: SLC29A1 Function and Localization

• SLC29A1 encodes the equilibrative nucleoside transporter 1 (ENT1), a member of the solute carrier family 29. It functions as a facilitative, Na+-independent uniporter for nucleosides and nucleobases, playing a key role in maintaining cellular homeostasis. Related references: PMID:8986748
• The transporter mediates the uptake of various nucleosides including adenosine, guanosine, inosine, uridine, thymidine, and cytidine, as well as purine and pyrimidine nucleobases such as hypoxanthine, adenine, guanine, thymine, and uracil. Related references: PMID:10722669, PMID:10755314, PMID:12527552, PMID:21795683, PMID:27995448
• SLC29A1 also transports nicotinamide, contributing to nicotinamide homeostasis and its biological roles. Related references: PMID:39885119
• In adipose tissue, ENT1 regulates inosine levels to control brown adipose tissue (BAT)-dependent energy expenditure. Related references: PMID:35790189
• The protein localizes to the basolateral and apical cell membranes, facilitating directional nucleoside flux in polarized cells like renal epithelia and Sertoli cells.
• ENT1 is widely expressed, with high levels in cerebral cortex, testis, erythrocytes, kidney, placenta, and small intestine. It is also found in adipose tissue and pancreas.
• According to UniProt, the protein is subject to glycosylation and phosphorylation, and multiple isoforms exist via alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200–300 of human SLC29A1. This region is within the intracellular loop, which may provide good accessibility for antibody binding in denaturing Western blot conditions.
• For Western blot, the predicted protein weight is approximately 50 kDa, although post-translational modifications such as glycosylation may result in a slightly higher apparent molecular weight.
• Cross-reactivity with mouse and rat has been indicated, enabling potential use in rodent models. Always validate specificity in the relevant species and tissue samples.
• For ELISA, appropriate coating and detection conditions should be optimized empirically. Consider using a known positive control.
• The antibody is shipped on ice bags; upon arrival, store at recommended temperature to maintain stability. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with high-quality IVD raw materials, including validated antibodies for targets like SLC29A1/ENT1 involved in nucleoside transport. We offer a comprehensive portfolio of polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to support your assay development needs. Whether you require rare-target sourcing or customized antibody production, our one-stop supply chain simplifies your path from concept to clinic.