Anti-SLC28A3 Polyclonal Antibody for WB, ELISA - Q9HAS3

Core Product Specifications and Parameters

Biological Background: SLC28A3 Function and Localization

• SLC28A3, also known as concentrative Na(+)-nucleoside cotransporter 3 (CNT3 or hCNT3), is encoded by the SLC28A3 gene and belongs to the solute carrier 28 family.
• It functions as a sodium-dependent transporter with broad selectivity for pyrimidine and purine nucleosides, mediating the uptake of physiological nucleosides such as thymidine, uridine, and inosine. Related references: PMID:11032837 PMID:15861042 PMID:16446384
• SLC28A3 exhibits transport characteristics of the N3/cib system with a 2:1 sodium/nucleoside coupling ratio. Related references: PMID:11032837
• The transporter also mediates uptake of several clinically relevant nucleoside analog drugs, including gemcitabine, AZT, ribavirin, and 3-deazauridine. Related references: PMID:11032837 PMID:17140564
• Subcellular localization studies indicate SLC28A3 is present at the cell membrane and also in the endoplasmic reticulum membrane.
• The protein is expressed in a variety of tissues, including pancreas, bone marrow, trachea, mammary gland, liver, prostate, and regions of intestine, brain, lung, placenta, testis, kidney, and heart.
• UniProt keywords highlight alternative splicing, proteomics identification, and a predicted 3D-structure, suggesting post-translational regulation and structural studies.
• The canonical molecular weight of SLC28A3 is approximately 77 kDa (calculated ~76.9 kDa), consistent with the protein weight provided.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 612–691), suggesting the antibody may detect full-length SLC28A3 and potentially C-terminal isoforms; consider validating in relevant sample systems.
• Suitable for WB and ELISA; optimizing antibody dilution for specific sample types (e.g., tissue lysates expressing SLC28A3) is recommended.
• Cross-reactivity is reported for human and mouse; verify specificity in your experimental model, especially when using isoforms or closely related family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-SLC28A3 pAb, bulk ancillary reagents, and custom sourcing for rare targets. For diagnostic manufacturers developing nucleoside transporter-related assays, CamelBio provides one-stop procurement support from concept to clinic, ensuring quality and supply chain continuity.