Anti-SLC28A2 Rabbit Polyclonal Antibody for WB & ELISA - O43868

Core Product Specifications and Parameters

Biological Background: SLC28A2 Function and Localization

• SLC28A2 (also known as CNT2, SPNT1) is a sodium-dependent, purine-selective nucleoside transporter. Related references: PMID:10087507, PMID:9435697
• It mediates concentrative uptake of purine nucleosides and uridine, characteristic of the N1/cif transport system. Related references: PMID:10087507, PMID:21795683, PMID:9435697
• SLC28A2 plays a critical role in purine nucleoside salvage in the kidney and other tissues. Related references: PMID:9435697
• The protein localizes to the apicolateral cell membrane, consistent with its role in epithelial transport.
• Expressed in heart, skeletal muscle, and other tissues (PubMed:9435697). In testis, localized to Sertoli cells and vascular endothelial cells (PubMed:35307651). Related references: PMID:9435697, PMID:35307651
• SLC28A2 is a phosphoprotein with multiple transmembrane helices, classified under solute carrier family 28.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region (aa 350–450) of human SLC28A2; the antibody is expected to recognize the native and denatured protein.
• For Western blot, consider a dilution range of 1:500–1:2000 and validate with positive lysates from kidney, heart, or testis tissues across human, mouse, and rat.
• A band at approximately 72 kDa is anticipated; use appropriate molecular weight markers and optimize transfer and blocking conditions.
• For ELISA, the antibody may be used as a capture or detection reagent; titrate from 0.5–2 μg/mL and pair with a compatible secondary antibody.
• Because SLC28A2 is a transmembrane protein, ensure mild detergent solubilization during sample preparation to preserve epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a single-channel solution for IVD raw-material procurement, from concept to clinic. For nucleoside transporter studies, we supply validated polyclonal antibodies such as this anti-SLC28A2 antibody, and can also develop customized monoclonal antibodies, optimize matched antibody pairs, and source bulk ancillary reagents. For rare-target requirements, our team provides tailored sourcing and technical consulting to accelerate your assay development.