Anti-SLC27A2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O14975

Core Product Specifications and Parameters

Biological Background: SLC27A2 Function and Localization

• Encodes long-chain fatty acid transport protein 2 (FATP2), a member of the solute carrier family 27 (SLC27A2). Also known as very long-chain acyl-CoA synthetase (VLACS).
• Facilitates import of long-chain fatty acids (LCFA) into cells and catalyzes their ATP-dependent conversion to fatty acyl-CoA, a key step preventing fatty acid efflux and driving uptake. Plays a pivotal role in regulating LCFA substrate availability for beta-oxidation and triglyceride synthesis in liver. Related references: PMID:10198260, PMID:10749848, PMID:11980911, PMID:20530735
• Activates very long-chain fatty acids and branched-chain fatty acids like phytanic acid and pristanic acid. Shows preference for n-3 fatty acids, generating CoA derivatives that are trafficked into phosphatidylinositol. Related references: PMID:10198260, PMID:21768100
• May contribute to synthesis of sphingosine-1-phosphate. Related references: PMID:24269233
• In vitro, activates THCA (a C27 bile acid precursor) but does not activate C24 bile acids; however, not critical for bile synthesis in vivo. Related references: PMID:11980911, PMID:20530735
• Located at endoplasmic reticulum membrane, peroxisome membrane, cell membrane, and microsome.
• Expressed in liver, kidney, placenta, intestine, brain, heart, and colon; predominantly in liver. A shorter isoform is expressed at lower levels in liver, placenta, and intestine. Related references: PMID:10198260, PMID:21768100, PMID:24269233
• Post-translational modifications include acetylation and phosphorylation; contains ATP-binding and transmembrane domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 290-573) of human FATP2; this region is conserved among species, consistent with observed cross-reactivity to mouse and rat.
• For Western blot, a band at approximately 70 kDa is expected; validate in the relevant sample system, such as liver lysate.
• In immunohistochemistry (paraffin), consider optimizing antigen retrieval and testing on liver or placenta sections.
• For immunofluorescence/ICC, permeabilization is required due to intracellular membrane localization; confirm with appropriate controls.
• Polyclonal antibodies may recognize multiple isoforms; verify specificity by using knockdown/knockout controls or other validation methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-SLC27A2 polyclonal antibody supports research in fatty acid metabolism and related disorders. Explore our validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your next project.