Anti-SLC27A1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q6PCB7

Core Product Specifications and Parameters

Biological Background: SLC27A1 Function and Localization

• Mediates long-chain fatty acid (LCFA) import at the plasma membrane and functions as an acyl-CoA ligase to convert LCFA and very-long-chain fatty acids into acyl-CoA esters, preventing efflux and promoting cellular fatty acid accumulation. Related references: PMID:12556534 PMID:20530735 PMID:21395585
• Localizes to the cell membrane, endomembrane system, and cytoplasm, supporting its dual role in transport and metabolic trapping.
• Highly expressed in muscle and adipose tissue, with lower levels in small intestine and brain gray matter; barely detectable in liver. Related references: PMID:10873384 PMID:21395585
• May regulate cholesterol metabolism (by similarity), linking LCFA handling to broader lipid homeostasis.
• Subject to alternative splicing, generating isoforms with potentially distinct functions or localizations.
• Belongs to the solute carrier family 27 (fatty acid transport protein family), characterized by nucleotide-binding and transmembrane domains.
• Key biological keywords include fatty acid metabolism, lipid transport, ligase activity, and membrane-associated transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 510–646 of human SLC27A1; epitopes in this C-terminal region should be recognized. Validate reactivity in the relevant species and tissue system.
• For Western blot, expect a band near 71 kDa using muscle or adipose tissue lysates as positive controls; consider using reducing/denaturing conditions.
• For immunofluorescence and immunocytochemistry, permeabilization is often required to access intracellular or membrane-associated pools; optimize fixation and blocking conditions.
• For ELISA, this polyclonal antibody may serve as a capture or detection reagent; pairing with validated monoclonal antibodies can improve assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. Our supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, all aligned with advanced lipid metabolism research. For SLC27A1-focused studies, this rabbit polyclonal antibody is supplied with full traceability, enabling reliable assay development and long-term research continuity.