Anti-SLC26A3 Polyclonal Antibody for WB and ELISA - P40879

Core Product Specifications and Parameters

Biological Background: SLC26A3 Function and Localization

• SLC26A3 (Down-regulated in adenoma protein DRA; CLD; solute carrier family 26 member 3) is a chloride anion exchanger belonging to the solute carrier 26 family.
• Mediates chloride-bicarbonate exchange with a 2:1 stoichiometry in intestinal epithelia (PubMed:16606687, PubMed:19321737, PubMed:22159084). Related references: PMID:16606687 PMID:19321737 PMID:22159084
• Plays a role in chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (By similarity).
• Localizes primarily to the apical cell membrane of epithelial cells.
• Expression is highest in the colon; expression is significantly decreased in adenomas (polyps) and adenocarcinomas.
• Functions as an antiporter and glycoprotein; classified under keywords such as Antiport, Cell membrane, Transport.
• Contains multiple transmembrane domains and is implicated in disease variants.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a recombinant immunogen spanning amino acids 505–764; consider verifying detection of full-length SLC26A3 (85 kDa) in relevant colon cell lines (e.g., Caco-2, HT-29) by Western blot.
• For ELISA applications, optimize the coating buffer and detection system using a recombinant SLC26A3 protein standard to ensure linearity and sensitivity.
• Due to the transmembrane nature of the target, include mild detergent in lysis buffers to improve extraction efficiency.
• Validate specificity by comparing signals in SLC26A3-positive and negative tissues; colon carcinoma tissues with down-regulated SLC26A3 may serve as a useful negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to a vast portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-SLC26A3 polyclonal antibody facilitates research into intestinal ion transport, colon cancer biomarker development, and reproductive biology. For scaling up your assay or sourcing rare-target reagents, our procurement platform bridges the gap from concept to clinic.