Anti-SLC26A2 Rabbit Polyclonal Antibody for WB and ELISA - P50443

Core Product Specifications and Parameters

Biological Background: SLC26A2 Function and Localization

• SLC26A2 (Solute Carrier Family 26 Member 2), also known as diastrophic dysplasia protein (DTD), is a sulfate transporter that mediates sulfate uptake into chondrocytes to maintain proteoglycan sulfation, which is essential for cartilage development (PubMed:11448940, PubMed:15294877, PubMed:20219950). Related references: PMID:11448940 PMID:15294877 PMID:20219950

• It functions as an electroneutral anion exchanger, mediating the exchange of sulfate ions for oxalate and chloride ions, and chloride ions for bromide, iodide, and nitrate ions (PubMed:20219950). Related references: PMID:20219950

• The coupling of sulfate transport to both hydroxyl and chloride ions likely ensures efficient uptake at acidic and alkaline pH, respectively (By similarity).

• The protein localizes to the cell membrane, particularly the apical membrane, and is ubiquitously expressed in human tissues, reflecting its fundamental role in sulfate homeostasis.

• SLC26A2 is a glycoprotein and phosphoprotein with multiple transmembrane helices; mutations in the gene are linked to diastrophic dysplasia and other skeletal disorders (keyword: Dwarfism).

• The mature protein has an apparent molecular weight of approximately 82 kDa, consistent with the predicted mass of 81.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-111), so the antibody may detect both full-length and potential N-terminal truncated isoforms; use appropriate positive controls.
• For Western blotting, expect a band near 82 kDa; validate with molecular weight markers and consider running reducing and non-reducing conditions.
• This antibody cross-reacts with mouse and rat SLC26A2, enabling cross-species studies; confirm reactivity in your specific experimental system.
• For ELISA, optimize coating antigen concentration and detection parameters; the antibody can be used in direct or indirect formats.
• Given SLC26A2’s role in sulfate transport, consider complementing antibody-based detection with functional sulfate uptake assays to correlate protein expression with activity.
• Store and ship on ice bags; avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For studies on sulfate transporters and cartilage biology, we offer this validated anti-SLC26A2 rabbit polyclonal antibody alongside optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and custom rare-target raw-material sourcing. Our integrated supply services help accelerate your assay development and ensure reliable supply continuity.