Anti-SLC25A4/ANT1 Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P12235

Core Product Specifications and Parameters

Biological Background: SLC25A4/ANT1 Function and Localization

• Protein identity and isoforms: SLC25A4 encodes the heart/skeletal muscle isoform of the ADP/ATP translocase (ANT1), a member of the mitochondrial carrier family. Alternative names include Adenine nucleotide translocator 1, ADP/ATP carrier protein 1, and Solute carrier family 25 member 4.
• Core antiporter function: Catalyzes the exchange of mitochondrial ATP for cytosolic ADP, a critical step in cellular energy supply. The carrier operates via an alternating access mechanism, cycling between cytoplasmic-open and matrix-open states to transport one substrate at a time. Related references: PMID:21586654 PMID:27693233 PMID:23173940
• Mitochondrial permeability transition pore (mPTP) involvement: Participates in the mitochondrial permeability transition pore, a non-specific channel that permeabilizes the inner mitochondrial membrane and triggers cell death. Related references: PMID:31883789
• Proton transporter and thermogenesis: In addition to nucleotide exchange, ANT1 can function as a proton transporter, thereby uncoupling respiration from ATP synthesis to generate heat. This proton-conducting activity is regulated by free fatty acids and is inhibited by ADP/ATP exchange, positioning ANT1 as a master regulator of energy output balance.
• Regulation of mitophagy: Independently of its transport activity, ANT1 promotes mitophagy by interacting with TIMM44, which inhibits the mitochondrial presequence translocase TIM23 and stabilizes PINK1 on damaged mitochondria.
• Subcellular localization: Resides exclusively in the mitochondrial inner membrane, a multi-spanning transmembrane protein.
• Post‑translational modifications and keywords: The protein is subject to acetylation, phosphorylation, methylation, and S‑nitrosylation. UniProtKB annotations highlight its roles in cardiomyopathy, progressive external ophthalmoplegia, and as a host factor in viral interactions.
• Tissue specificity: Expressed in erythrocytes as well as cardiac and skeletal muscle (as indicated by its isoform name).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide targeting the central region (aa 100–200) of human SLC25A4. The epitope lies in a conserved region; cross-reactivity to mouse and rat is supported by sequence alignment. Validate reactivity with your specific model species if critical.
• For Western blotting, a major band at ~33 kDa is expected. KO‑validated control lysates (e.g., HeLa or A549) can confirm specificity and avoid off‑target signals.
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), the staining pattern should be cytoplasmic with a mitochondrial distribution. Antigen retrieval with citrate buffer (pH 6.0) may improve detection. Include appropriate isotype controls.
• The antibody is suitable for immunoprecipitation (IP) and ELISA, making it versatile for interaction studies and quantitative assays. When using IP, consider cross‑linking the antibody to beads to avoid co‑elution of the heavy chain at ~55 kDa.
• For functional assays that perturb mitochondrial integrity, note that ANT1 can influence mPTP opening; thus, staining may be affected by the cellular stress state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with ready access to validated IVD raw materials. This SLC25A4/ANT1 antibody complements a comprehensive portfolio covering mitochondrial metabolism and bioenergetics targets. Beyond individual antibodies, CamelBio supplies optimized antibody pairs, bulk‑format ancillary reagents, and customized sourcing solutions for rare or difficult‑to‑produce proteins, helping you transition seamlessly from research to clinical application.