Anti-SLC25A38 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96DW6

Core Product Specifications and Parameters

Biological Background: SLC25A38 Function and Localization

• SLC25A38, also known as appoptosin or mitochondrial glycine transporter GlyC, is a member of the solute carrier family 25 and functions as a glycine transporter across the mitochondrial inner membrane.
• It plays a critical role in heme biosynthesis by importing glycine into the mitochondrial matrix, where glycine is condensed with succinyl-CoA to form 5-aminolevulinate (ALA), the first committed step of heme synthesis.
• The protein is essential during erythropoiesis, facilitating the high demand for heme in developing red blood cells.
• In addition to its transport function, SLC25A38 has been identified as a pro-apoptotic protein capable of inducing caspase-dependent apoptosis.
• Subcellular localization studies confirm its residence in the mitochondrial inner membrane as a multi-pass transmembrane protein.
• Tissue expression profiling indicates preferential expression in erythroid cells, with lower levels in other tissues.
• Keywords associated with SLC25A38 include disease variant, membrane, mitochondrion, mitochondrial inner membrane, and transport, reflecting its roles in human physiology and pathology.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-100) of human SLC25A38. As a rabbit polyclonal antibody, it may recognize multiple epitopes; validate specificity in your experimental system using knockdown or knockout controls if available.
• For Western blotting, the predicted molecular weight is 34 kDa; however, post-translational modifications or endogenous protein forms may appear at slightly different sizes. Consider using erythroid cell lines (e.g., K562) as positive controls.
• In immunofluorescence/ICC, mitochondrial localization should appear punctate. Co-staining with a mitochondrial marker (e.g., MitoTracker or anti-Tom20) is recommended to confirm specificity.
• For ELISA applications, this antibody can be used to detect recombinant or purified SLC25A38 protein. Optimize coating and detection conditions with the specific antigen.
• Cross-reactivity is reported for human and mouse; if using in other species, sequence homology analysis is advised before testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated IVD raw-material partner, connecting diagnostic developers with high-quality reagents like this anti-SLC25A38 polyclonal antibody, suitable for mitochondrial transporter and heme biosynthesis research. Our comprehensive portfolio includes validated antibodies, antigen proteins, and ancillary reagents to accelerate your assay development. For customized requirements or bulk orders, our sourcing infrastructure supports rare targets and large-scale supply with technical consultation at every stage.