Anti-SLC25A27 Polyclonal Antibody for WB, IF/ICC, ELISA - O95847

Core Product Specifications and Parameters

Biological Background: SLC25A27 (UCP4) Function and Localization

• SLC25A27, also known as mitochondrial uncoupling protein 4 (UCP4), belongs to the solute carrier family 25 and is implicated in mitochondrial transmembrane transport.
• Facilitates proton transport across the inner mitochondrial membrane, dissipating the proton gradient and potentially protecting against oxidative and metabolic stress at neuronal synapses.
• Regulates glutamate-induced proton conductance in astrocytes, shifting energy metabolism toward aerobic glycolysis and lactate transfer to neurons for ATP synthesis.
• Can transport chloride ions with lower efficiency, though the exact transport mechanism remains to be elucidated.
• Localized predominantly to the mitochondrial inner membrane, consistent with its role in mitochondrial uncoupling.
• Expressed mainly in adult and fetal brain tissues, with low levels in spinal cord, corpus callosum, and substantia nigra.
• Contains transmembrane helices and repeats characteristic of mitochondrial carrier proteins; subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 201-300 of human SLC25A27 (NP_004268.3). For Western blot, consider a predicted band at approximately 36 kDa; validate with appropriate positive controls.
• For immunofluorescence/ICC, anticipate mitochondrial localization; use appropriate fixation and permeabilization methods for mitochondrial antigens.
• This antibody is designated for WB, IF/ICC, and ELISA. Optimize dilution and conditions in the specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing reliable access to validated antibodies, reagents, and technical support for mitochondrial research. Our SLC25A27 rabbit polyclonal antibody, along with our extensive portfolio, enables efficient development of diagnostic assays and research tools. We also offer customized sourcing for rare targets and bulk ancillary reagents to accelerate your project from concept to clinic.